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05-623 Anti-RNA polymerase II Antibody, clone CTD4H8

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05-623
200 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Yeast (S. cerevisiae)WB, ChIP-seqMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-623
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-RNA polymerase II Antibody, clone CTD4H8
      Background InformationRNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
      Product Information
      FormatPurified
      Control
      • A431 nuclear extract.
      PresentationPurified mouse IgG1 in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
      Quality LevelMQ100
      Applications
      ApplicationAnti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.
      Key Applications
      • Western Blotting
      • ChIP-seq
      Biological Information
      ImmunogenPeptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
      CloneCTD4H8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Phospho- and non-phospho-RNA polymerase II.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Yeast (S. cerevisiae)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G Chromatography
      UniProt Number
      Molecular Weight210-220 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on HeLa nuclear extracts.

      Western Blot Analysis:
      0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Global Trade Item Number
      Catalogue Number GTIN
      05-623 04053252511127