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04-1545 Anti-RARα Antibody, clone 9α-9A6

Anti-RARα Antibody, clone 9α-9A6 is a Mouse Monoclonal Antibody for detection of RARα also known as Retinoic acid receptor alpha polypeptide & has been validated in WB, ICC.

Anti-RARα Antibody, clone 9α-9A6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: NR1B1, RAR, RARA, RAR-alpha UniProt Number: P10276
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04-1545
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMAscitesMonoclonal Antibody
      Description
      Catalogue Number04-1545
      DescriptionAnti-RARα Antibody, clone 9α-9A6
      Alternate Names
      • Nuclear receptor subfamily 1 group B member 1
      • Retinoic acid receptor, alpha polypeptide
      • nucleophosmin-retinoic acid receptor alpha fusion protein
      • NPM-RAR long form
      • retinoic acid receptor, alpha
      Background InformationRetinoic acid receptor alpha (RARa) is a nuclear receptor encoded by the RARA gene. This gene is sometimes referred to as NR1B1 (nuclear receptor subfamily 1, group B, member 1). RARa is a member of the steroid/thyroid superfamily of nuclear receptor proteins that regulate gene expression by binding to RNA and other elements of DNA response. As implied by its name, this protein is a receptor for retinoic acid, a metabolite of vitamin A. Retinoic acid is necessary for normal organogenesis, but can act as a teratogen when present at high levels during embryonic and fetal development. During fetal and embryonic development retinoic acid regulates transcription through the activation of nuclear receptors, specifically the Retinoic Acid Receptor (RAR). Signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR), which form RXR/RAR heterodimers. There are three isoforms associated with RAR; RARα, RARβ and RARγ.
      References
      Product Information
      FormatAscites
      Control
      • HL60 cell lysate
      PresentationUnpurified mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-RARα Antibody, clone 9α-9A6 is a Mouse Monoclonal Antibody for detection of RARα also known as Retinoic acid receptor alpha polypeptide & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected RARα in HeLa and A431 cells.
      Biological Information
      ImmunogenRecombinant protein corresponding to human RARα.
      EpitopeUnknown
      Cloneclone 9α-9A6
      HostMouse
      SpecificityThis antibody recognizes RARα.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with human. Homology to mouse and rat is 91%.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryRetinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 7468032]).[supplied by OMIM].
      Gene Symbol
      • NR1B1
      • RAR
      • RARA
      • RAR-alpha
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: This is a receptor for retinoic acid. This metabolite has profound effects on vertebrate development. Retinoic acid is a morphogen and is a powerful teratogen. This receptor controls cell function by directly regulating gene expression.

      SIZE: 462 amino acids; 50771 Da

      SUBUNIT: Interacts with CDK7 (By similarity). Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NOCA7 in a ligand- inducible manner.

      SUBCELLULAR LOCATION: Nucleus.

      DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.

      PTM: Phosphorylated. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity).

      DISEASE: Chromosomal aberrations involving RARA may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM.

      SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
      Molecular Weight58 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HL60 cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected RARα on 10 µg of HL60 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      04-1545 04053252408793

      Documentation

      Anti-RARα Antibody, clone 9α-9A6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-RARα Antibody, clone 9α-9A6 Certificates of Analysis

      TitleLot Number
      Anti-RAR, clone 9-9A6 - 1990513 1990513
      Anti-RAR, clone 9-9A6 - NRG1718895 NRG1718895
      Anti-RARα, clone 9α-9A6 - 2391412 2391412
      Anti-RARα, clone 9α-9A6 - 2443059 2443059
      Anti-RARα, clone 9α-9A6 - 3180474 3180474
      Anti-RARα, clone 9α-9A6 - 3277106 3277106
      Anti-RARα, clone 9α-9A6 - 3594291 3594291
      Anti-RARα, clone 9α-9A6 - 3891416 3891416
      Anti-RARα, clone 9α-9A6 -2526830 2526830
      Anti-RARα, clone 9α-9A6 -2812317 2812317

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies