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MABS1696 Anti-RANKL Antibody, clone 6A12.1

Anti-RANKL, clone 6A12.1, Cat. No. MABS1696, is a mouse monoclonal antibody that detets RANKL in human cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-RANKL Antibody, clone 6A12.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: TNFSF11, OPGL, RANKL, TRANCE UniProt Number: O14788
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MABS1696
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MUnpurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1696
      DescriptionAnti-RANKL Antibody, clone 6A12.1
      Alternate Names
      • Tumor necrosis factor ligand superfamily member 11
      • Osteoclast differentiation factor
      • ODF
      • Osteoprotegerin ligand
      • OPGL
      • Receptor activator of nuclear factor kappa-B ligand
      • TNF-related activation-induced cytokine
      • CD254
      Background InformationTumor necrosis factor ligand superfamily member 11 (UniProt: O14788; also known as Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254) is encoded by the TNFSF11 (also known as OPGL, RANKL, TRANCE) gene (Gene ID: 8600) in human. RANKL is a single-pass type II membrane protein that is found in high levels in the peripheral lymph nodes and in lower levels in the spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Its levels are shown to be up-regulated by T-cell receptor stimulation. RANKL has a cytoplasmic domain (aa 1-47), a helical domain (aa 48-68) and an extracellular domain (aa 69-317). RANKL has been identified to affect the immune system and control bone regeneration and remodeling. RANKL is a cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. It is shown to augment the ability of dendritic cells to stimulate naive T-cell proliferation and is an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. It is shown to induce osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells. RANKL is also reported to activate Akt through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which suggests a role of RANKL in the regulation of cell apoptosis. Three isoforms of RANKL have been described that are produced by alternative splicing. Defects in TNFS11 gene can cause osteoporosis, both a severe autosomal recessive form as well a benign autosomal dominant form.
      References
      Product Information
      FormatUnpurified
      PresentationMouse monoclonal antibody in tissue culture supernatant without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-RANKL, clone 6A12.1, Cat. No. MABS1696, is a mouse monoclonal antibody that detets RANKL in human cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected RANKL in human tonsil tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 95 amino acids from the C-terminal region of human RANKL.
      Clone6A12.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6A12.1 detects RANKL protein in human cells. It targets an epitope within 95 amino acids from the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TNFSF11
      • OPGL
      • RANKL
      • TRANCE
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~36 kDa observed; 35.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with RANKL recombinant protein.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected human recombinant RANKL protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1696 04054839355851