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MABS1942-25UG Anti-Perilipin-1 Antibody, clone Peri 112.17.1

Anti-Perilipin-1, clone Peri 112.17.1, Cat. No. MABS1942, is a mouse monoclonal antibody that detects Perilipin-1 and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

MABS1942-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS1942-25UG
      DescriptionAnti-Perilipin-1 Antibody, clone Peri 112.17.1
      Alternate Names
      • Lipid droplet-associated protein
      Background InformationPerilipin-1 (UniProt: O60240; also known as Lipid droplet-associated protein) is encoded by the PLIN1 (also known as PERI, PLIN) gene (Gene ID: 5346) in human. Perilipins are lipid droplet (LD)- binding proteins that are required for optimal lipid storage and fatty acid release. In mammals the perilipin family of comprises five members: perilipin, adipophilin, TIP47, S3-12 and MLDP/OXPAT (also named PLIN1-5. Perilpin-1 is a modulator of adipocyte lipid metabolism that is detected in adipocytes and is also shown to be expressed in hepatocytes of human steatotic liver. It coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase. It is shown to play a role in unilocular lipid droplet formation by activating cell death inducing DFFA like effector c (CIDEC). Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. In adipocytes, perilipin-1 can undergo phosphorylation by protein kinase A that changes its conformation and permits lipolysis by hormone-sensitive lipase. Perilipin knockout mice are lean and display aberrant adipocyte lipolysis, enhanced leptin production, and resistance to diet-induced obesity. Mutations in PLIN1 gene are linked to familial lipodystrophy that is characterized by loss of subcutaneous adipose tissue primarily affecting the lower limbs, insulin-resistant diabetes mellitus, hypertriglyceridemia, and hypertension. (Ref.: Straub, BK et al. (2008). Hepatology. 47(6); 1936-1946; Tansey JT., et al. (2004). IUBMB Life. 56(7); 379-385).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Perilipin-1, clone Peri 112.17.1, Cat. No. MABS1942, is a mouse monoclonal antibody that detects Perilipin-1 and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Electron Microscopy
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Perilipin-1 in Immunoprecipitation applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Perilipin-1 in human breast and lipoma tissue sections.

      Electron Microscopy Analysis: A representative lot detected Perilipin- positive lipid droplets by Immunoelectron Microscopy applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).

      Immunocytochemistry Analysis: A representative lot detected Perilipin-1 in Immunocytochemistry applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).

      Western Blotting Analysis: A representative lot detected Perilipin-1 in Western Blotting applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to first 20 amino acids from the N-terminal region of human Perilipin-1.
      ClonePeri 112.17.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone Peri 112.17.1 is a mouse monoclonal antibody that detects Perilipin-1. It targets an epitope within 20 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Bovine
      • Human
      Species Reactivity NoteHuman, Bovine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PLIN1
      • PERI
      • PLIN
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~ 65 KDa observed; 55.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3/L1 cell lysate.

      Western Blotting Analysis: A 1:500 Dilution of this antibody detected Perilipin-1 in NIH3T3/L1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1942-25UG 04061841276715

      Documentation

      Anti-Perilipin-1 Antibody, clone Peri 112.17.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Perilipin-1 Antibody, clone Peri 112.17.1 Certificates of Analysis

      TitleLot Number
      Anti-Perilipin-1, clone Peri 112.17.1 - Q3329774 Q3329774

      References

      Reference overviewPub Med ID
      On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex.
      Heid, H; Rickelt, S; Zimbelmann, R; Winter, S; Schumacher, H; Dörflinger, Y; Kuhn, C; Franke, WW
      PLoS One  9  e90386  2014

      Show Abstract       		24587346 24587346