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PH01 Anti-PTP1B (Ab-1) Mouse mAb (FG6-1G)

This Anti-PTP1B (Ab-1) Mouse mAb (FG6-1G) is validated for use in Immunoblotting for the detection of PTP1B (Ab-1).

Anti-PTP1B (Ab-1) Mouse mAb (FG6-1G) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Protein Tyrosine Phosphatase 1B

PH01
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      OverviewRecognizes the ~50 kDa PTPase 1B protein. Weak reactivity with mouse PTPase 1B and human TC/PTPase (a 48 kDa protein).

      This product has been discontinued.
      Catalogue NumberPH01
      Brand Family Calbiochem®
      SynonymsAnti-Protein Tyrosine Phosphatase 1B
      References
      ReferencesFlint, A.J., et al. 1993. EMBO J. 12, 1937.
      Brown-Shimer, S., et al. 1992. Cancer Res. 52, 478.
      Fischer, E.H., et al. 1991. Science 253, 401.
      Pallen, C.J., et al. 1991. Biochem. J. 276, 315.
      Brown-Shimer, S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 5148.
      Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
      Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
      Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlBSC-40 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Application CommentsThis antibody is weakly reactive to murine PTPase 1B and human TC PTPase (~48 kDa). PTPaseB1 undergoes reversible phosphorylation during mitosis; this is accompanied by a mobility shift on SDS-PAGE. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human protein tyrosine phosphatase 1B
      Immunogenhuman
      Epitopewithin the catalytic domain
      CloneFG6-1G
      HostMouse
      IsotypeIgG2a
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PH01 0

      Documentation

      Anti-PTP1B (Ab-1) Mouse mAb (FG6-1G) Certificates of Analysis

      TitleLot Number
      PH01

      References

      Reference overview
      Flint, A.J., et al. 1993. EMBO J. 12, 1937.
      Brown-Shimer, S., et al. 1992. Cancer Res. 52, 478.
      Fischer, E.H., et al. 1991. Science 253, 401.
      Pallen, C.J., et al. 1991. Biochem. J. 276, 315.
      Brown-Shimer, S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 5148.
      Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
      Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
      Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.

      Brochure

      Title
      Protein Phosphatases Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-October-2007 RFH
      SynonymsAnti-Protein Tyrosine Phosphatase 1B
      ApplicationImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing mouse splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~50 kDa PTPase 1B protein.
      BackgroundProtein tyrosine phosphatases (PTPases) make up an expanding family of transmembrane and intracellular proteins, which function to remove phosphate groups from protein phosphoryl-tyrosine residues. PTPase 1B was initially identified as an active intracellular phosphatase from human placenta made up of 321 amino acids. It was subsequently shown to derive from a larger precursor protein containing 435 amino acids. Further examination failed to show alternative splicing or more than a single copy of the PTP1B in the genome. Purification of a 50,000 kDa protein with phosphatase activity from human placenta demonstrated the protein to have an amino acid composition similiar to that for PTPase 1B and N-terminal analysis revealed an identical sequence. PTPases are involved in regulating processes where protein kinases and protein tyrosine phosphorylation are important. Recently it has been demonstrated that expression of PTPase 1B has profound effects on the transformation induced by the human neu oncogene. Based on this and other observations it has been hypothesized that PTPases have the potential to function as tumor suppressor genes.
      HostMouse
      Immunogen specieshuman
      Immunogenrecombinant, human protein tyrosine phosphatase 1B
      Epitopewithin the catalytic domain
      CloneFG6-1G
      IsotypeIgG2a
      Speciesavian, hamster, human, mouse (weakly), rat
      Positive controlBSC-40 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody is weakly reactive to murine PTPase 1B and human TC PTPase (~48 kDa). PTPaseB1 undergoes reversible phosphorylation during mitosis; this is accompanied by a mobility shift on SDS-PAGE. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesFlint, A.J., et al. 1993. EMBO J. 12, 1937.
      Brown-Shimer, S., et al. 1992. Cancer Res. 52, 478.
      Fischer, E.H., et al. 1991. Science 253, 401.
      Pallen, C.J., et al. 1991. Biochem. J. 276, 315.
      Brown-Shimer, S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 5148.
      Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
      Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
      Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.