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MABF2654-25UG Anti-PGRMC1 Antibody, clone 108-B6

MABF2654-25UG
25 μg  
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      Overview

      Description
      Catalogue NumberMABF2654-25UG
      DescriptionAnti-PGRMC1 Antibody, clone 108-B6
      Alternate Names
      • Membrane-associated progesterone receptor component 1
      • mPR
      Background InformationMembrane-associated progesterone receptor component 1 (UniProt: O00264; also known as mPR, PGRMC1) is encoded by the PGRMC1 (also known as HPR6.6, PGRMC) gene (Gene ID: 10857) in human. PGRMC1 is a single pass homodimeric membrane protein that is widely expressed with highest expression observed in liver and kidney. It is also shown to be overexpressed in multiple types of cancers and can serve as an important biomarker of their proliferative state. It has short N-terminal extracellular domain (aa 1-24), a transmembrane domain (aa 25-43), and a cytoplasmic domain (aa 44-195). PGRMC1 is a component of a progesterone-binding protein complex with many cellular functions. It is required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan. It also serves as an intracellular heme chaperone that regulates heme synthesis and acts as a heme donor for some hemoproteins. PGRMC1 contains a cytochrome b5 heme-binding domain (aa 72-171) that lacks the conserved iron-binding histidine residue at positions 107 and 131. PGRMC1 is reported to suppress p53 and Wnt/-catenin pathway to promote self-renewal of pluripotent stem cells (PSCs) and inhibit their differentiation. Its levels are rapidly down regulated during early differentiation of PSCs. (Ref.: Kim, JY et al. (2018). Sci. Rep. 3; 3048; Kim, JY., et al. (2019). Sci. Rep. 9; 653).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-PGRMC1, clone 108-B6, Cat. No. MABF2654, is a mouse monoclonal antibody that detects Membrane-associated progesterone receptor component 1 (PGRMC1) and is tested for use in Flow Cytometry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected PGRMC1 in Western Blotting applications (Kim, J.Y., et. al. (2018). Sci Rep. 8(1):3048; Kim, J.Y., et. al. (2019). Sci Rep. 9(1):653).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated PGRMC1 in Immunoprecipitation applications (Kim, J.Y., et. al. (2018). Sci Rep. 8(1):3048).

      Flow Cytometry Analysis: A representative lot detected PGRMC1 in Flow Cytometry applications (Kim, J.Y., et. al. (2018). Sci Rep. 8(1):3048; Kim, J.Y., et. al. (2019). Sci Rep. 9(1):653; Choi, H.S., et. al. (2008). Cell Tissue Res. 333(2):197-206).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenH9 cells cultured with retinoic acid.
      EpitopeC-terminal
      Clone108-B6
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 108-B6 is a mouse monoclonal antibody that detects human Membrane-associated progesterone receptor component 1 (PGRMC1). It targets an epitope within 13 amino acids from the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PGRMC1
      • HPR6.6
      • PGRMC
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight21.67 kDa calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HepG2 cells.

      Flow Cytometry Analysis: 2 µg of this antibody detected PGRMC1 in one million HepG2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2654-25UG 04061842626168