Millipore Sigma Vibrant Logo

ST1204 Anti-PGC-1α Rabbit pAb

This Anti-PGC-1α Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of PGC-1α.

Anti-PGC-1α Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
ST1204
View Pricing & Availability

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
ST1204-100UL
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 100 ul
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      OverviewRecognizes the ~113 kDa PGC-1α protein in BAT cells.
      Catalogue NumberST1204
      Brand Family Calbiochem®
      Application Data
      Detection of mouse PGC-1α by immunoprecipitation followed by immunoblotting.
      Samples:
      Lane 1: Whole tissue extract (200 µg) from brown adipose tissue of a mouse held at room temperature, immunoprecipitated with 2 µg Anti-PGC-1α Rabbit pAb (Cat. No. ST1204).
      Lane 2: Whole tissue extract (200 µg) from brown adipose tissue of a mouse held at 4°C for 5 hr, immunoprecipitated with 2 µg Anti-PGC-1α Rabbit pAb (Cat. No. ST1204).
      Lane 3: Whole cell lysate from CHO cells overexpressing NT-PGC-1α positive control.
      Lane 4: Whole cell lysate from COS cells overexpressing PGC-1α positive control.
      Lane 5: Whole tissue extract (200 µg) from brown adipose tissue of a mouse held at 4°C for 5 hr, immunoprecipitated using Rabbit IgG negative control.
      Lane 6: Whole tissue extract (10% input) from brown adipose tissue of a mouse held at room temperature (no IP).
      Lane 7: Whole tissue extract (10% input) from brown adipose tissue of a mouse held at 4°C for 5 hr (no IP).
      Antibody for immunoblotting: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesChang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
      Zhang, Y., et al. 2009. J. Biol. Chem. 284, 32813.
      Lai, L., et al. 2008. Genes Dev. 14, 1948.
      Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
      Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
      Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
      Product Information
      FormLiquid
      FormulationIn 50 mM PBS
      Positive controlBrown adipose tissue from mice exposed to cold
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoprecipitation
      Not Western Blot
      Application NotesImmunoblotting (not recommended)
      Immunoprecipitation (1-2 µg/100-200 µg total protein)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenA recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
      ImmunogenMouse
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Regulatory Review
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1204-100UL 04055977224108

      Documentation

      Anti-PGC-1α Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PGC-1α Rabbit pAb Certificates of Analysis

      TitleLot Number
      ST1204

      References

      Reference overview
      Chang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
      Zhang, Y., et al. 2009. J. Biol. Chem. 284, 32813.
      Lai, L., et al. 2008. Genes Dev. 14, 1948.
      Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
      Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
      Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-February-2011 RFH
      ApplicationImmunoblotting (not recommended)
      Immunoprecipitation (1-2 µg/100-200 µg total protein)
      Application Data
      Detection of mouse PGC-1α by immunoprecipitation followed by immunoblotting.
      Samples:
      Lane 1: Whole tissue extract (200 µg) from brown adipose tissue of a mouse held at room temperature, immunoprecipitated with 2 µg Anti-PGC-1α Rabbit pAb (Cat. No. ST1204).
      Lane 2: Whole tissue extract (200 µg) from brown adipose tissue of a mouse held at 4°C for 5 hr, immunoprecipitated with 2 µg Anti-PGC-1α Rabbit pAb (Cat. No. ST1204).
      Lane 3: Whole cell lysate from CHO cells overexpressing NT-PGC-1α positive control.
      Lane 4: Whole cell lysate from COS cells overexpressing PGC-1α positive control.
      Lane 5: Whole tissue extract (200 µg) from brown adipose tissue of a mouse held at 4°C for 5 hr, immunoprecipitated using Rabbit IgG negative control.
      Lane 6: Whole tissue extract (10% input) from brown adipose tissue of a mouse held at room temperature (no IP).
      Lane 7: Whole tissue extract (10% input) from brown adipose tissue of a mouse held at 4°C for 5 hr (no IP).
      Antibody for immunoblotting: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.
      DescriptionProtein G purified rabbit polyclonal antibody. Recognizes the ~113 kDa PGC-1α protein.
      BackgroundPGC-1α is a co-activator of PPARα, PPARγ, and other transcription factors that regulate the transcriptonal program of adaptive thermogenesis in brown adipose tissue, hepatic/renal gluconeogenesis, and muscle fiber type switching. The full length protein is detected at 113 kDa and is induced in BAT by cold exposure, in liver and kidney by fasting, and in skeletal muscle by exercise. Alternative splicing of the full length gene produces a 270 aa N-terminal splice variant that migrates at ~38 kDa and is induced by the same physiological signals which induce expression of the full length protein. The N-terminal PGC-1α splice variant is also subject to post translational modifications which alter its migration and apparent molecular weight.
      HostRabbit
      Immunogen speciesMouse
      ImmunogenA recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlBrown adipose tissue from mice exposed to cold
      FormLiquid
      FormulationIn 50 mM PBS
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Regulatory Review
      ReferencesChang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
      Zhang, Y., et al. 2009. J. Biol. Chem. 284, 32813.
      Lai, L., et al. 2008. Genes Dev. 14, 1948.
      Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
      Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
      Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.