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ST1203 Anti-PGC-1α Mouse mAb (1F3.9)

This Anti-PGC-1α Mouse mAb (1F3.9) is validated for use in Immunoblotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation for the detection of PGC-1α.

Anti-PGC-1α Mouse mAb (1F3.9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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ST1203
  
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      Overview

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RMMonoclonal Antibody
      Description
      OverviewRecognizes the ~113 kDa PGC-1α protein in COS cells.
      Catalogue NumberST1203
      Brand Family Calbiochem®
      Application Data
      Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: DAB.

      Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: fluorescence.

      Detection of mouse PGC-1α by immunoblotting. Sample: Whole tissue extract from brown adipose tissue of mice held at room temperature (left 3 lanes) and mice held at 4°C for 5 hr (right three lanes). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesChang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
      Zhang, Y. et al. 2009. J. Biol. Chem. 284, 32813.
      Lai, L., et al. 2008. Genes Dev. 14, 1948.
      Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
      Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
      Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
      Product Information
      FormLiquid
      FormulationIn 50 mM PBS
      Positive controlBrown adipose tissue from mice exposed to cold
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml)
      Immunocytochemistry (5-10 µg/ml)
      Immunohistochemistry (5-10 µg/ml)
      Immunoprecipitation (1:100)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenA recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
      ImmunogenMouse
      Clone1F3.9
      HostMouse
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Regulatory Review
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      ST1203 0