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09-796 Anti-PADI4 Antibody, CT

Anti-PADI4 Antibody, C-terminus is an antibody against PADI4 for use in WB, ELISA.

Anti-PADI4 Antibody, CT MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: PADI4, PAD, PAD4, PADI5, PDI4, PDI5 UniProt Number: Q9UM07
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09-796
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISARbPurifiedPolyclonal Antibody
      Description
      Catalogue Number09-796
      DescriptionAnti-PADI4 Antibody, CT
      Alternate Names
      • HL-60 PAD
      • PADI-H protein
      • Peptidylarginine deiminase IV
      • Protein-arginine deiminase type IV
      • peptidyl arginine deiminase, type IV
      • peptidyl arginine deiminase, type V
      • protein-arginine deiminase
      • Protein-arginine deiminase type-4
      Background InformationPADI4 (Protein-arginine deiminase type-4) is a member of a family of enzymes responsible for the conversion of arginine residues to citrulline residues via catalysis of the deimination of the arginine residues. PADI4 down-regulates histone H3 and H4 arginine methylation, both by preventing arginine methylation by CARM1 and HRMT1L2/PRMT1 and by converting methylarginine to citrulline. This protein may play a role in granulocyte and macrophage development leading to inflammation and immune response.
      References
      Product Information
      FormatPurified
      Control
      • HEK293 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing PBS and 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PADI4 Antibody, C-terminus is an antibody against PADI4 for use in WB, ELISA.
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesELISA: A 1:1000 dilution of a previous lot was used on ELISA.
      Biological Information
      ImmunogenKLH-conjugated synthetic linear peptide corresponding to the C-terminal region of PADI4.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody recognizes PADI4 at and around the C-terminus.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response.
      [provided by RefSeq].
      Gene Symbol
      • PADI4
      • PAD
      • PAD4
      • PADI5
      • PDI4
      • PDI5
      Purification MethodSaturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
      UniProt Number
      UniProt SummaryFUNCTION: Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.

      CATALYTIC ACTIVITY: Protein L-arginine + H2O = protein L-citrulline + NH3.

      COFACTOR: Binds 5 calcium ions per subunit.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasmic granule. Note= Cytoplasmic granules of eosinophils and neutrophils.

      TISSUE SPECIFICITY: Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.

      INVOLVEMENT IN DISEASE: PADI4 is thought to be a rheumatoid arthritis susceptibility locus [MIM:180300]. Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.

      SEQUENCE SIMILARITIES: Belongs to the protein arginine deiminase family.

      BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters:

      KM=0.055 mM for fibrinogen

      KM=0.064 mM for filaggrin

      Vmax=33.2 µmol/h/mg enzyme toward fibrinogen

      Vmax=8.0 µmol/h/mg enzyme toward filaggrin

      pH dependence:

      Optimum pH is 6.5-9.0.
      Molecular Weight~74 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on HEK293 cell lysate.

      Western Blotting Analysis: 1:25-1:500 dilution of this antibody detected PADI4 in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC from date of receipt. For long term storage, store at -20ºC
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      09-796 04053252737237

      Documentation

      Anti-PADI4 Antibody, CT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PADI4 Antibody, CT Certificates of Analysis

      TitleLot Number
      Anti-PAD4, C-terminus - NG1611692 NG1611692
      Anti-PADI4, C-terminus - 2463629 2463629
      Anti-PADI4, C-terminus - 2266796 2266796
      Anti-PADI4, C-terminus - NG1939042 NG1939042
      Anti-PADI4, C-terminus -2608743 2608743
      Anti-PADI4, C-terminus -2657455 2657455
      Anti-PADI4, C-terminus Polyclonal Antibody 2976879
      Anti-PADI4, C-terminus Polyclonal Antibody 2966320
      Anti-PADI4, C-terminus_2837184 2837184

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies