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MABE2036-100UG Anti-PADI2/PAD2 Antibody, clone DN2

Anti-PADI2/PAD2, clone DN2, Cat. No. MABE2036, is a mouse monoclonal antibody that detects Protein-arginine deiminase type-2 (PADI2) and is tested in ELISA, Immunohistochemistry, Multiplexing, and Wes

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      Overview

      Description
      Catalogue NumberMABE2036-100UG
      DescriptionAnti-PADI2/PAD2 Antibody, clone DN2
      Alternate Names
      • Peptidylarginine deiminase 2
      • PAD2
      Background InformationProtein-arginine deiminase (UniProt: A0A5F9DB16; also known as PADI2, PAD2) is encoded by the PADI2 gene (Gene ID: 100357368) in rabbit. Protein-arginine deiminases (PAD) catalyze the hydrolysis of peptidyl-arginine to peptidyl-citrulline and alter protein function. Five isoforms of PAD have been described in mammals (PAD1, 2, 3, 4, and 6) that are homologous in their catalytic site but are expressed differently in different cells and tissues and have different physiological roles. PAD2, one of the most abundant PADs has three domains: N-terminal domain (aa 1-114), a central domain (aa 116-274), and a C-terminal domain (aa 285-657). High calcium concentration is reported to be a prerequisite for PAD2 activation. A redox potential maintains its catalytic site in a reduced state. Overexpression of PAD2 has been associated with inflammatory diseases, such as rheumatoid arthritis and neurodegenerative diseases including multiple sclerosis. Higher levels of PAD2 are observed in transformed cell model of breast cancer progression with increasing tumor malignancy and in a murine models of xenograft tumors. Clone DN2 binds to an epitope in the N-terminal region and is ideally suited for quantitative ELISA. (Ref.: Wang, Y., et al. (2021). Int. J. Cancer. 148(2); 267-276; Vejlstrup, A., et al. (2019). J. Inflamm. Res. 12; 137 144; Damgaard, D., et al. (2016). Rheumatology 55(5); 918-927; Damgaard, D., et al. (2014). J. Immunol. Methods. 405; 15-22).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-PADI2/PAD2, clone DN2, Cat. No. MABE2036, is a mouse monoclonal antibody that detects Protein-arginine deiminase type-2 (PADI2) and is tested in ELISA, Immunohistochemistry, Multiplexing, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Multiplexing
      • ELISA
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected PADI2/PAD2 in Immunohistochemistry applications (Damgaard, D., et al. (2014). J Immunol Methods. 405:15-22).

      Western Blotting Analysis: A representative lot detected PADI2/PAD2 in Western Blotting applications (Damgaard, D., et al. (2014). J Immunol Methods. 405:15-22; Bawadekar, M., et al. (2017). J Autoimmun. 80:39-47).

      ELISA Analysis: A representative lot detected PADI2/PAD2 in ELISA applications (Damgaard, D., et al. (2014). J Immunol Methods.;405:15-22; Damgaard, D., et al. (2016). Rheumatology (Oxford). 55(5):918-27).

      Multiplexing: A representative lot detected PADI2/PAD2 in Multiplexing applications (Vejlstrup, A., et al. (2019). J Inflamm Res. 29:12:137-144).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenProtein-arginine deiminase (PADI2/PAD2) from rabbit skeletal muscle.
      EpitopeN-terminal
      CloneDN2
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone DN2 is a mouse monoclonal antibody that detects Protein-arginine deiminase 2 (PADI2/PAD2). It targets an epitope within the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rabbit based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PADI2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~80 kDa observed; 74.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with full-length, recombinant, Human PADI2/PAD2.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, recombinant, Human PADI2/PAD2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABE2036-100UG 04065271058965