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MABF2697-100UG Anti-P. aeruginosa serogroup It-6 Antibody, clone PTA-5386

Anti-P. aeruginosa serogroup It-6, clone PTA-5386, Cat. No. MABF2697, is a mouse monoclonal antibody that detects LPS from Pseudomonas aeruginosa (It-6) and is tested in ELISA.

NewMABF2697-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2697-100UG
      DescriptionAnti-P. aeruginosa serogroup It-6 Antibody, clone PTA-5386
      Alternate Names
      • Pseudomonas aeruginosa It-6 serotype
      Background InformationPseudomonas aeruginosa is a gram-negative, aerobic, non-spore forming bacteria that causes a variety of infections, particularly in immunocompromised subjects. Following infection, the airway mucosal immune system rapidly develops a pro-inflammatory response, leading to production of inflammatory mediators both in aerial compartment and local tissue initiated by the recognition of pathogen-associated molecular patterns (PAMPs) by specific Toll-like receptors and triggered by the epithelial cells and the resident immune cells. Pathogenesis is mediated by several virulence factors, including motility, adherence to biotic and abiotic surfaces, and secreted toxins. These factors modulate or disrupt host cells signaling pathways, target extracellular matrix, and induce tissue damage. Mouse monoclonal antibody, clone PTA-5386, developed against serotype It-6 directed to LPS O-side chain is opsonic for uptake and killing of bacteria by PMN and is shown to protect against fatal sepsis in mice rendered neutropenic with cyclophosphamide and challenged with homologous P. aeruginosa. (Ref.: Secher T., et al. (2011). J. Antimicrob. Chemother. 66(5); 1100 1109; Lai, Z., et al. (2005). Vaccine. 23(25); 3264-3271).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2 in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-P. aeruginosa serogroup It-6, clone PTA-5386, Cat. No. MABF2697, is a mouse monoclonal antibody that detects LPS from Pseudomonas aeruginosa (It-6) and is tested in ELISA.
      Key Applications
      • ELISA
      • Inhibition
      Application NotesTested Applications

      ELISA Analysis: A representative lot detected LPS from Pseudomonas aeruginosa (It-6) in ELISA applications (Lai, Z., et al. (2005). Vaccine. 23(25):3264-71).


      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHeat killed Pseudomonas aeruginosa of It-6 serotype.
      EpitopeUnknown
      ClonePTA-5386
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone PTA-5386 is a mouse monoclonal antibody that detects lipopolysaccharide (LPS) from It-6 serotype of Pseudomonas aeruginosa.
      IsotypeIgG2κ
      Species Reactivity
      • Bacteria
      Species Reactivity NotePseudomonas sp.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightN/A
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA with LPS specific for serogroup It-6.

      ELISA Analysis: Serial dilutions of this antibody detected LPS from Pseudomonas aeruginosa serogroup It-6.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2697-100UG 04065271059009