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MAB328-I-25UG Anti-Oligodendrocytes Antibody, clone CE-1

Anti-Oligodendrocytes, clone CE-1, Cat. No. MAB328-I, is a mouse monoclonal antibody that detects Oligodendrocytes and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

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MAB328-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB328-I-25UG
      DescriptionAnti-Oligodendrocytes Antibody, clone CE-1
      Alternate Names
      • Myelin-oligodendrocyte glycoprotein
      Background InformationOligodendrocytes are large glial cells found in the central nervous system. They are responsible for production of myelin sheath that insulates neuronal axons. The first step in the generation of oligodendrocytes is the emergence of oligodendrocyte precursors (OPCs) from embryonic radial glia. Subsequently, OPCs differentiate into immature oligodendrocytes before they begin to generate myelin. This process is regulated by both intrinsic and extrinsic signals in cells. Oligodendrocytes have only a brief period for myelination early during differentiation, and mature oligodendrocytes are incapable of myelinating. An essential signal for the onset of myelination is reported to be provided by the electrical activity of neurons. Although the ATP released from axons firing axon potentials does not directly act on OPCs or oligodendrocytes, it triggers the release of leukemia inhibitory factor (LIF) from astrocytes, which promotes myelination. Although the electrical activity of neurons is an essential promyelinating factor, other factors also come into play in this process. During development, all growing nerve fibers are shown to express the polysialylated-neural cell adhesion molecule (PSA-NCAM) that can prevent homophilic NCAM adhesion and cell-cell interactions. When this form of NCAM is downregulated, as it is in electrically active neurons, only then the process of myelination can proceed. (Ref.: Bradl, M., and Lassmann, H. (2010). Acta Neuropathol. 119(1); 37-53; Berto, S., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(48); 24334-24342).
      References
      Product Information
      FormatPurified
      Control
      • MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94
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      PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Oligodendrocytes, clone CE-1, Cat. No. MAB328-I, is a mouse monoclonal antibody that detects Oligodendrocytes and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Western Blotting
      Application NotesTested applications

      Western Blotting Analysis: A representative lot detected Oligodendrocytes in Western Blotting application (Goolsby, J., et al. (2003). Proc Natl Acad Sci USA. 100(25):14926-31).

      Immunocytochemistry Analysis: A representative lot detected Oligodendrocytes in Immunocytochemistry application (Goolsby, J., et al. (2003). Proc Natl Acad Sci USA. 100(25):14926-31; Glavaski-Joksimovic, A., et al. (2009). Neuroscience. 162(2):472-81).

      Immunofluorescence Analysis: A representative lot detected Oligodendrocytes in Immunofluorescence applications (Dubreuil, C., et al. (2003). J Cell Biol. 162(2):233-43; Glavaski-Joksimovic, A., et al. (2009). Neuroscience. 162(2):472-81; Wuthrich, C., et al. (2012). J Neuropathol Exp Neurol. 71(1):54-65).

      Immunohistochemistry Applications: A representative lot detected Oligodendrocytes in Immunohistochemistry applications (Dubreuil, C., et al. (2003). J Cell Biol. 162(2):233-43; Glavaski-Joksimovic, A., et al. (2009). Neuroscience. 162(2):472-81; Wuthrich, C., et al. (2012). J Neuropathol Exp Neurol. 71(1):54-65).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenRat glial cell membrane proteins followed by rat CNS white matter.
      EpitopeUnknown
      CloneCE-1
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone CE-1 is a mouse monoclonal antibody that detects oligodendrocytes.
      IsotypeIgM
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodLigatrap
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in Rat oligodendrocytes.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Rat Oligodendrocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB328-I-25UG 04065269048244