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MABE1799 Anti-Nrf2 Antibody, clone 103

Anti-Nrf2, clone 103 , Cat. No. MABE1799, is a highly specific rat monoclonal antibody that targets Nuclear factor erythroid 2-related factor 2 and has been tested in Immunohistochemistry and Western Blotting.

Anti-Nrf2 Antibody, clone 103 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Nfe2l2, Nrf-2, Nrf2 UniProt Number: Q60795
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MABE1799
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MWB, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1799
      DescriptionAnti-Nrf2 Antibody, clone 103
      Alternate Names
      • Nuclear factor erythroid 2-related factor 2
      • NF-E2-related factor 2
      • NFE2-related factor 2
      • Nuclear factor
      • erythroid derived 2
      • like 2
      Background InformationNuclear factor erythroid 2-related factor 2 (UniProt: Q60795; also known as Nrf2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, like 2) is encoded by the Nfe2l2 (also known as Nrf-2, Nrf2) gene (Gene ID: 18024) in murine species. Nrf2 is a cap n collar basic-region leucine zipper transcription factor that is involved in adaptation of cells to oxidative stress and electrophiles. It bind to antioxidant response (ARE) elements in the promoter regions of target genes and induces a set of cytoprotective genes that are involved in the synthesis of reduced glutathione (GSH), repair of oxidized protein thiols, scavenging of reactive oxygen species, and drug metabolism. It is cytosolic under unstressed conditions, but translocates into the nucleus upon induction by electrophilic agents. Under normal unstressed conditions it is rapidly turned over in a Keap-1 dependent manner through Cul3-Rbx1 ubiquitination and proteasomal degradation. Phosphorylation of Ser40 by protein kinase C in response to oxidative stress dissociates Nrf2 from its Keap-1 and promotes its translocation into the nucleus. It is widely expressed with highest expression in liver, skeletal muscle, luminal cells of the stomach and intestine, lining of the bronchi and alveoli, and in renal tubules; followed by heart, spleen, testis and brain. Nrf2 is constitutively upregulated in many tumors due to somatic mutations in the Keap1 or Nrf2 genes. Tumors overexpressing Nrf2 exhibit increased resistance to chemotherapeutic drugs and higher rates of proliferation. (Ref.: Hayes, JD., and Ashford, MLJ (2012). Cell Metab. 16(2): 139-141).
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nrf2, clone 103 , Cat. No. MABE1799, is a highly specific rat monoclonal antibody that targets Nuclear factor erythroid 2-related factor 2 and has been tested in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A representative lot detected Nrf2 in Immunohistochemistry applications (Saito, R., et. al. (2015). Mol Cell Biol. 36(2):271-84).

      Western Blotting Analysis: A representative lot detected Nrf2 in Western Blotting applications (Maruyama, A., et. al. (2008). Arch Biochem Biophys. 477(1):139-45; Kobayashi, E.H., et. al. (2016). Nat Commun. 7:11624; Taquchi, K., et. al. (2016). Toxicol Sci. 152(1):40-52; Saito, R., et. al. (2015). Mol Cell Biol. 36(2):271-84; Taquchi, K., et. al. (2014). Mol Cell Biol. 34(5):900-13).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 179 amino acids from internal region of murine Nuclear factor erythroid 2-related factor 2.
      Clone103
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 103 detects Nrf2 in Keap-1 knockout cells. It targets an epitope within 179 amino acids in the internal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteMouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nfe2l2
      • Nrf-2
      • Nrf2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~67 kDa observed; 66.90 kDa calculated Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Keap1-KO MEF cell lysates versus Nrf2-KO MEF cell lysates.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nrf2 in Keap1-KO MEF cell lysates versus Nrf2-KO MEF cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1799 04054839197857