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MABN1814 Anti-Neurogenin-2 Antibody, clone 7G4

Anti-Neurogenin-2, clone 7G4, Cat. No. MABN1814, is a highly specific mouse monoclonal antibody that targets Neurogenin-2 and has been tested in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin).

Anti-Neurogenin-2 Antibody, clone 7G4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Neurog2, Ath4a, Atoh4, Ngn2 UniProt Number: P70447
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MABN1814
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MIF, IH(P), ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1814
      DescriptionAnti-Neurogenin-2 Antibody, clone 7G4
      Alternate Names
      • Neurogenin-2
      • Helix-loop-helix protein mATH-4A
      • mATH4A
      • NGN-2
      • Protein atonal homolog 4
      Background InformationNeurogenin-2 (UniProt P70447; also known as Helix-loop-helix protein mATH-4A, mATH4A, NGN-2, Protein atonal homolog 4) is encoded by the Neurog2 (also known as Ath4a, Atoh4, Ngn2) gene (Gene ID 11924) in murine species. Neuronal differentiation is largely under the control of basic Helix-Loop-Helix (bHLH) proneural transcription factors that play key roles during development of the embryonic nervous system. Neurogenin-2 (NGN-2) is a bHLH proneural transcription factor that orchestrates the progression from neural progenitor to differentiating neuron in several regions of the central nervous system, including the formation of sensory neurons from the epibranchial placode and neurogenesis in dorsal root ganglia. NGN-2 dimerizes with TCF3 (E12/E47), the heterodimer then binds to target DNA regions and recruit transcriptional coactivators, such as p300/CBP and Brg1, to facilitate the transcription of a cascade of additional bHLH transcription factors, such as NeuroD, as well as structural and functional genes associated with mature neuronal activity.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neurogenin-2, clone 7G4, Cat. No. MABN1814, is a highly specific mouse monoclonal antibody that targets Neurogenin-2 and has been tested in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin).
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected neurogenin-2 in mouse small intestine and colon tissue sections.

      Immunocytochemistry Analysis: A representative lot detected NGN2-positive cells in dissociated neural tube (dNT) cultured under 10 ng/mL BMP2 for 48 hrs by fluorescent immunocytochemistry (Lo, L., et al. (2002). Development. 129(7):1553-1567).

      Immunofluorescence Analysis: A representative lot detected NGN2 immunoreactivity among a small subset of cells at the dorsal margin of the neural tube and among a greater number of SOX10-positive cells in the neural crest migration pathway by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed E10.5 rat spinal cord section (Lo, L., et al. (2002). Development. 129(7):1553-1567).
      Biological Information
      ImmunogenGST-tagged mouse neurogenin-2 N-terminal fragment (Lo, L., et al. (2002). Development. 129(7):1553-1567).
      Clone7G4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThe specific staining by clone 7G4 was blocked by NGN2-GST fusion, but not by NGN1-fusion protein (Lo, L., et al. (2002). Development. 129(7):1553-1567).
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteMouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Neurog2
      • Ath4a
      • Atoh4
      • Ngn2
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight28.22/28.06 kDa (mouse/rat) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse cerebral cortex tissue section.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected neurogenin-2 in mouse cerebral cortex tissue section.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1814 04054839081316