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MABN1853 Anti-Neuregulin-2 Antibody (NRG2), clone 8D11

Anti-Neuregulin-2 (NRG2), clone 8D11, Cat. No. MABN1853, is a highly specific mouse monoclonal antibody that targets Neuregulin-2 (NRG2) and has been tested in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Nrg2 UniProt Number: P56974
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MABN1853
200 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, IF, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1853
      DescriptionAnti-Neuregulin-2 Antibody (NRG2), clone 8D11
      Alternate Names
      • Pro-neuregulin-2, membrane-bound isoform
      • Pro-NRG2
      • Neuregulin-2
      • NRG2
      • Divergent of neuregulin 1
      • DON-1
      Background InformationPro-neuregulin-2, membrane-bound isoform (UniProt P56974; also known as Pro-NRG2) is encoded by the Nrg2 gene (Gene ID 100042150) in murine species. The ErbB4 receptor is widely expressed in GABAergic interneurons and reply on the engagement of neuregulins (NRGs) as ligands for signaling during neural processes in the adolescent and adult central nervous system (CNS). NRG1 transcript is generally lower than NRG2 in postnatal CNS and NRG2 is a major functional ErbB4 ligand in the postnatal brain. NRGs are synthesized as single-pass transmembrane pro-forms that is posttranslationally converted to signalling ligands by extracellular sheddases. The presence of an extracellular immunoglobulin (Ig)-like domain allows NRG1/2 ectodomain to accumulate near the site of cleavage through interactions with extracellular matrix heparan proteoglycans. NMDARs are required to release the NRG2 ectodomain from the surface of interneuron cell bodies. Stimulation of ErbB4 by the NRG2 ectodomain not only promotes the internalization of ion channels from the cell surface, but also the association of ErbB4 with GluN2B-containing NMDARs, resulting in a rapid downregulation of NMDARs on inhibitory interneurons, but not pyramidal neurons.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neuregulin-2 (NRG2), clone 8D11, Cat. No. MABN1853, is a highly specific mouse monoclonal antibody that targets Neuregulin-2 (NRG2) and has been tested in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin).
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      Application NotesImmunocytochemistry Analysis: A representative lot detected somatodendritic pro-NRG2 puncta on the surface of cultured rat hippocampal ErbB4+ interneurons (DIV 21 & 28) fixed with 4% paraformaldehyde without permeabilization (Vullhorst, D., et al. (2015). Nat. Commun. 6:7222).

      Immunofluorescence Analysis: A representative lot detected NRG2 immunoreactivity among ErbB4+ interneurons in CA1 strata pyramidale and radiatum of 4% paraformaldehyde-fixed, 0.25% Triton X-100-permeabilized rat brain sections (Vullhorst, D., et al. (2015). Nat. Commun. 6:7222).
      Biological Information
      ImmunogenRecombinant mouse mature Neuregulin-2.
      Clone8D11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 8D11 epitope lies within neuregulin-2, which corresponds to the extracellular domain of membrane-bound pro-neuregulin-2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nrg2
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight80.30/67.13/70.64 (human pro-neuregulin-2 isoform 1/DON-1B/DON-1R), 32.60/19.43/22.94 (human neuregulin-2 isoform 1/DON-1B/DON-1R), 80.17/80.04 kDa (mouse pro-neuregulin-2 isoform NRG2-16A/DON-1M), 32.66/32.53/34.24/28.66 kDa (mouse neuregulin-2 isoform NRG2-16A/DON-1M/DON-1S (NRG2-5)/NRG2-10), 80.79/79.84/79.97/79.01/28.63/34.34/79.96 kDa (rat pro-neuregulin-2 isoform NTAK-alpha1/NTAK-alpha2A/NTAK-alpha2B (NTAK-alpha2-1P)/NTAK-beta/NTAK-gamma/NRG2-alpha/NRG2-beta), 33.46/32.50/32.63/31.67/28.63/34.34/32,63 kDa (rat neuregulin-2 isoform NTAK-alpha1/NTAK-alpha2A/NTAK-alpha2B (NTAK-alpha2-1P)/NTAK-beta/NTAK-gamma/NRG2-alpha/NRG2-beta) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human cerebellum tissue sections.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Purkinje cells surface Neuregulin-2 (NRG2) immunoreactivity in human cerebellum tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1853 04054839081392