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ABE2875 Anti-Nek6

Anti-Nek6, Cat. No. ABE2875, is a highly specific rabbit polyclonal antibody that targets Serine/threonine-protein kinase Nek6 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-Nek6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: NEK6 UniProt Number: Q9HC98
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ABE2875
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, IH(P), IFRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2875
      DescriptionAnti-Nek6
      Alternate Names
      • Serine/threonine-protein kinase Nek6
      • EC: 2.7.11.1
      • Never in mitosis A-related kinase 6
      • NimA-related protein kinase 6
      • Protein kinase SID6-1512
      Background InformationSerine/threonine-protein kinase Nek6 (UniProt: Q9HC98; also known as EC: 2.7.11.1, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512) is encoded by the NEK6 gene (Gene ID: 10783) in human. Nek6, a member of the NIMA-related serine/threonine kinase family that plays an important role in mitotic cell cycle progression and is required for chromosome segregation at metaphase-anaphase transition. It localizes to spindle microtubules in metaphase and anaphase and to the mid-body during cytokinesis. Nek6 is ubiquitously expressed, with highest expression in heart and skeletal muscle. Its levels are up-regulated in a variety of malignant cancers, such as breast, colon, lung, and gastric cancers. Nek6 is shown to be directly phosphorylated by Chk1 and Chk2, suggesting that it is a direct target of the DNA damage checkpoint. It is also phosphorylated upon IR or UV-induced DNA damage. Nek6 is activated by phosphorylation at Ser206. Although expression of Nek6 is tightly controlled in normal cells, this protein is highly overexpressed in various human cancers and regulates tumor progression and its expression and activity levels directly correlate to tumorigenicity of human cancer cells. Inhibition of Nek6 can lead to apoptosis. (Ref.: Nassirpour, R et al. (2010). Mol. Cancer Res. 8(5); 717-28).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nek6, Cat. No. ABE2875, is a highly specific rabbit polyclonal antibody that targets Serine/threonine-protein kinase Nek6 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Nek6 in human pancreas and human cervical cancer tissue.

      Immunofluorescence Analysis: A representative lot detected Nek6 in HeLa cells that were transfected with Nek6 siRNA (O'Regan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90).

      Western Blotting Analysis: A representative lot detected Nek6 in IP blot from lysates prepared from untransfected HEK 293 cells synchronized in S or M phase of the cell cycle with hydroxyurea or nocodazole, HeLa cells were either mock transfected or transfected (O'Regan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90; O'Regan, L., et. al. (2015). J Cell Biol. 209(3):349-58).

      Immunoprecipitation Analysis: A representative lot detected Nek6 in lysates prepared from untransfected HEK 293 cells synchronized in S or M phase of the cell cycle with hydroxyurea or nocodazole (O'Regan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90; O'Regan, L., et. al. (2015). J Cell Biol. 209(3):349-58).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the first 15 amino acids from the N-terminal region of human Serine/threonine-protein kinase Nek6.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Serine/threonine-protein kinase Nek6 in human cells. It targets an epitope with in the first 15 amino acids in the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEK6
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~35 kDa observed; 35.71 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HT1080 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Nek6 in 10 µg of HT1080 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2875 04054839315978