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MABN2311-100UL Anti-NUMB Antibody, clone Ab21

Anti-NUMB, clone Ab21, Cat. No. MABN2311, is a mouse monoclonal antibody that detects NUMB and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

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MABN2311-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2311-100UL
      DescriptionAnti-NUMB Antibody, clone Ab21
      Alternate Names
      • Protein numb homolog
      • h-Numb
      • Protein S171
      Background InformationProtein numb homolog (UniProt: P49757; also known as h-Numb, Protein S171) is encoded by the NUMB (also known as C14orf41) gene (Gene ID: 8650) in human. NUMB is an evolutionarily conserved endocytic protein that make the daughter cells different after asymmetric divisions by segregating to only one daughter. NUMB is a peripheral membrane protein present in cell and endosome membranes. It is required throughout embryonic neurogenesis to maintain neural progenitor cells by allowing their daughter cells to choose progenitor over neuronal cell fate. However, they are not required for the proliferation of neural progenitor cells before the onset of neurogenesis. It is also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. NUMB is shown to interact with AP-2 and clathrin-coated pits via -adaptin protein. It undergoes phosphorylation at serine 276 and 295 by CAMK1, which decouples it from the recycling machinery and results in inhibition of its interaction with AP-2 and clathrin-mediated endocytosis. NUMB also functions as an oncosuppressor by inhibiting Notch signaling and stabilizing p53. This effect is dependent upon its interaction with Mdm2 that ubiquitinates p53 and commits it to proteasomal degradation. Nine isoforms of NUMB have been described that are produced by alternative splicing. (Ref.: Colaluca, IN., et al. (2018). J. Cell Biol. 217(2); 745-762; Tasoni, D., et al. (2015). J. Cell. Biol. 211(4); 845-862; Tang, H., et al. (2005). Mol. Cell. Biology 25(8); 2899-2909; Tokomitsu H., et al. (2005). J. Biol. Chem. 280(42); 35108-35118).
      References
      Product Information
      FormatPurified
      Control
      • MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94
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      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-NUMB, clone Ab21, Cat. No. MABN2311, is a mouse monoclonal antibody that detects NUMB and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunofluorescence
      Application NotesTested applications

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected NUMB in A549 and U87MG cell lysates.

      Immunofluorescence Analysis: A representative lot detected NUMB in Immunofluorescence application (Colaluca, I.N., et al. (2008). Nature. 451(7174):76-80; Tosoni, D., et al. (2015). J Cell Biol. 211(4):845-62; Santoro, A., et al. (2019). Cell Rep. 26(3):624-638.e8).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated NUMB in Immunoprecipitation application (Colaluca, I.N., et al. (2008). Nature. 451(7174):76-80; Colaluca, I.N., et al. (2018). J Cell Biol. 217(2):745-762).

      Western Blotting Analysis: A representative lot detected NUMB in Western Blotting application (Colaluca, I.N., et al. (2008). Nature. 451(7174):76-80; Tosoni, D., et al. (2015). J Cell Biol. 211(4):845-62; Tosoni, D., et al. (2017). EMBO Mol Med. 9(5):65-671; Colaluca, I.N., et al. (2018). J Cell Biol. 217(2):745-762).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human NUMB protein.
      EpitopeC-terminal half
      CloneAb21
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone Ab21 is a mouse monoclonal antibody that detects Protein numb homolog (NUMB). It targets an epitope within 15 amino acids from the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Monkey based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NUMB
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~70 kDa observed; 70.8 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Neuro2a cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected NUMB in Neuro2a cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2311-100UL 04065269049272