Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).
More>>This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2). Less<<
Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.
Catalogue Number
PC138
Brand Family
Calbiochem®
Synonyms
Anti-Nuclear Factor ΚB
References
References
Kolberg, R., 1997. J. NIH Research9, 29. Van Antwerp, D.J. et al. 1996. Science274, 787. Beg, A.A., et al. 1995. Nature376, 167. Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141. Wulczyn, F.G., et al. 1992. Nature358, 597.
Enzyme-Linked Immunosorbent Assay Gel Shift Immunoblotting (Western Blotting) Immunoprecipitation
Application Notes
ELISA (see comments) Gel Shift (see comments) Immunoblotting (1:500, chemiluminescence) Immunoprecipitation (see comments)
Application Comments
This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH
Immunogen
Human
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb Certificates of Analysis
Title
Lot Number
PC138
References
Reference overview
Kolberg, R., 1997. J. NIH Research9, 29. Van Antwerp, D.J. et al. 1996. Science274, 787. Beg, A.A., et al. 1995. Nature376, 167. Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141. Wulczyn, F.G., et al. 1992. Nature358, 597.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-October-2007 RFH
Synonyms
Anti-Nuclear Factor ΚB
Application
ELISA (see comments) Gel Shift (see comments) Immunoblotting (1:500, chemiluminescence) Immunoprecipitation (see comments)
NFκB (Nuclear factor κB) is a transcription factor that plays crucial roles in gene regulation including those involved in immune and inflammatory responses. Active NFκB is most commonly composed of the heterodimer DNA binding subunits p50 and p65 (RelA). These subunits are members of a multigene family called the rel family. In addition to p50 and RelA, three other proteins are implicated in dimer formation leading to transcriptional activation, p52, c-rel and rel B. Inhibitors to this process exist including another member of the rel family IκBα which binds to c-rel, p65 and relB preventing DNA binding by their homo- or heterodimers. It has recently been shown that NFκB plays a role in the apoptotic process - when RelA, the gene that codes for an NFκB subunit, was knocked out, this lead to the death of mouse embryos through apoptosis of the liver cells. Similarly, it has been shown in a wide range of cells that NFκB which has been inactivated by IκBα were more sensitive to TNF-α-induced apoptosis than control cells. Evidence exists for NFκB playing both anti- and pro-apoptotic roles - it has been suggested that different combinations of subunits may result in different outcomes in terms of cell survival.
Host
Rabbit
Immunogen species
Human
Immunogen
a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH
Isotype
IgG
Species
human
Positive control
Daudi cells
Form
Liquid
Formulation
In 5 mM sodium phosphate buffer, pH 7.4.
Preservative
≤0.1% sodium azide
Comments
This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Kolberg, R., 1997. J. NIH Research9, 29. Van Antwerp, D.J. et al. 1996. Science274, 787. Beg, A.A., et al. 1995. Nature376, 167. Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141. Wulczyn, F.G., et al. 1992. Nature358, 597.
