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444105 Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11)

This Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) is validated for use in Immunoblotting, IF, IHC, IP for the detection of Mannose 6-Phosphate Receptor.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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444105
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, MkMMonoclonal Antibody

Pricing & Availability

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444105-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~300 kDa mannose 6-phosphate receptor protein.
      Catalogue Number444105
      Brand Family Calbiochem®
      References
      ReferencesDintzis, S., et al. 1994. J. Biol. Chem. 269, 12159.
      Dintzis, S., and Pfeffer, S. 1990. EMBO J. 9, 77.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunohistochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml, non-reducing, see comments)
      Immunofluorescence (see comments)
      Immunohistochemistry (2-5 µg/ml)
      Immunoprecipitation (see comments)
      Application CommentsDoes not detect MPR in hamster (CHO) cells. Not suitable for immunoblotting using reducing conditions. This antibody has also been reported to work for immunoprecipitation and immunofluorescence. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogennative bovine cation independent (CI)-mannose 6-phosphate receptor
      ImmunogenBovine
      Epitopewithin the extracelluar domain
      Clone2G11
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      444105-100UG 04055977205411

      Documentation

      Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) Certificates of Analysis

      TitleLot Number
      444105

      References

      Reference overview
      Dintzis, S., et al. 1994. J. Biol. Chem. 269, 12159.
      Dintzis, S., and Pfeffer, S. 1990. EMBO J. 9, 77.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2007 RFH
      ApplicationImmunoblotting (1 µg/ml, non-reducing, see comments)
      Immunofluorescence (see comments)
      Immunohistochemistry (2-5 µg/ml)
      Immunoprecipitation (see comments)
      DescriptionPurified mouse monoclonal antibody. Recognizes the mannose 6-phosphate receptor protein.
      BackgroundLysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of ~300 kDa cation-independent MPRs. The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPRs is predominantly late endosomes, fewer in the trans Golgi network, and ~10% at cell surface. In addition to its man6P binding activity, MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An ~240 kDa soluble, truncated form of the extracellular domain of MPR has been detected circulating in serum that is capable of binding both ligands.
      HostMouse
      Immunogen speciesBovine
      Immunogennative bovine cation independent (CI)-mannose 6-phosphate receptor
      Epitopewithin the extracelluar domain
      Clone2G11
      IsotypeIgG2a
      Speciesbovine, not hamster, human, monkey
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not detect MPR in hamster (CHO) cells. Not suitable for immunoblotting using reducing conditions. This antibody has also been reported to work for immunoprecipitation and immunofluorescence. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesDintzis, S., et al. 1994. J. Biol. Chem. 269, 12159.
      Dintzis, S., and Pfeffer, S. 1990. EMBO J. 9, 77.