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IM35 Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5)

Overview

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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IM35-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~55 kDa latent and the ~43 kDa active forms of MMP-1.
      Catalogue NumberIM35
      Brand Family Calbiochem®
      Application Data
      Detection of human MMP-1 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts CCD-41SK cells. Primary antibody: Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5) (Cat. No. IM35) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB 7, 1434.
      Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1.
      Woessner, J.F. 1991. FASEB 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1; 99-106.
      Product Information
      DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1. Frozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen sections (see application references)
      Immunoblotting (1 µg/ml)
      Paraffin sections (2.5 µg/ml, pressure cooker pre-treatment required)
      Application CommentsTo prepare conditioned medium for positive control, incubate HT-1080 cells for 2 h at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Does not cross-react with bovine. Will not cross-react with human MMP-2, MMP-3, MMP-8, MMP-9, or MMP-13. For best results with paraffin sections, pre-treat with a pressure cooker; however, heat, saponin or trypsin can be used in place of a pressure cooker. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (VQGQNVLHGYPKDIYSSFG) corresponding to amino acids 332-350 of human MMP-1
      ImmunogenHuman
      Clone41-1E5
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      IM35-100UG 04055977220568