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ABE1851 Anti-MLL-3

Anti-MLL3, Cat. No. ABE1851, is a rabbit polyclonal antibody that detects Myeloid-lineage leukemia protein 3 homolog (MLL3) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

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ABE1851
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE1851
      DescriptionAnti-MLL-3
      Alternate Names
      • HALR
      • Histone-lysine N-methyltransferase 2C
      • EC: 2.1.1.43
      • Lysine-N-methyltransferase 2C
      • Myeloid-lineage leukemia protein 3 homolog
      Background InformationHistone-lysine N-methyltransferase 2C (UniProt: Q8BRH4; also known as EC:2.1.1.354, Lysine N-methyltransferase 2C, Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog) is encoded by the Kmt2c (also known as Mll3) gene (Gene ID: 231051) in murine species. MLL 3 is a histone methyltransferase that catalyzes the methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of lysine 4 of histone H3 (H3K4). It is a part of chromatin remodeling machinery that predominantly forms H3K4me1 methylation marks at active chromatin sites where transcription and DNA repair take place. It is a DHHC-type zinc finger protein with eight PHD-type zinc fingers, a SET domain (aa 4763-4879), a post-SET domain (aa 4887-4903) and a RING-type zinc finger (aa 343-388). The SET domain interacts with histone H3, but not H2A, H2B, and H4. In adults, MLL-3 is detected in testis, kidney, spleen, and lung and is weakly expressed in brain. It is absent in heart and liver tissue. Mll3 gene is reported to be frequently mutated in cancers and shows a high frequency of loss-of-function mutations, which are spread over the entire protein, including the PHD fingers and SET domain. Mutations in Mll3 gene are reported to cause defects in BRCA1 associated protein 1 (BAP1) recruitment to the enhancers of tumor-suppressor genes, which represses enhancer activity and promotes tumorigenesis. Depletion of MLL3 can enhance cell migration, but does not elevate the proliferation rate of cancer cells. (Ref.: Zheng, J-Y., et al. (2021). Cell Death Dis. 12(4); Article 364).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MLL3, Cat. No. ABE1851, is a rabbit polyclonal antibody that detects Myeloid-lineage leukemia protein 3 homolog (MLL3) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Western Blotting
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:250-1,000 dilution from a representative lot detected MLL3 in mouse testis and mouse kidney tissue sections.

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected mouse recombinant MLL3.

      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MLL3 in U2OS cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of murine Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog (MLL3).
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog (MLL3). It targets an epitope within 18 amino acids from the N-terminal half.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Kmt2C
      • Mll3
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~540 kDa observed; 540.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in rat uterus tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected MLL3 in rat uterus tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1851 04054839233210

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