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MABE1987-25UL Anti-LINE1 ORF2 Antibody, clone 1G4E11

Anti-LINE1 ORF2, clone 1G4E11, Cat. No. MABE1987, is a mouse monoclonal antibody that detects LINE-1 retrotransposable element ORF2 protein and is tested for use in ELISA, Inhibition Assay, and Western Blotting.

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MABE1987-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1987-25UL
      DescriptionAnti-LINE1 ORF2 Antibody, clone 1G4E11
      Alternate Names
      • EC:2.7.7.49
      • ORF2p endonuclease
      • ORF2p
      Background InformationLINE-1 retrotransposable element ORF2 protein (UniProt: O00370; also known as EC:2.7.7.49, ORF2p endonuclease, ORF2p) is encoded by the Orf2p gene (Gene ID: 107987054) in human. Full length long interspersed element-1 (L1) is an autonomous non-long terminal repeat retrotransposon that contains two open reading frames encoding ORF1p and ORF2p proteins. L1 is reported to be responsible for all of the retrotransposon-induced genomic instability in the human genome and is the only active source of the functional ORF1 and ORF2 proteins required for mobilization of LINEs, SINEs, and SVA elements. The human ORF2p, a 149 kDa protein, contains three domains: an N-terminal endonuclease domain, a reverse transcriptase domain, and C-terminal domain with putative RNA binding activity. Its endonuclease activity allows the introduction of nicks in the chromosomal target DNA and is known to cleave DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to sites of LINE-1 integration in the genome. Human and mouse ORF2p is reported to exhibit a high degree of sequence homology. Clone 1G4E11 can recognize sequences within the endonuclease domain of the human ORF2 protein. It exhibits specificity to the human ORF2p and can detect the full-length and truncated ORF2 proteins expressed in mammalian cells. (Ref.: Sokolowski M., et al. (2014). Mob DNA. 5(1); 29; Feng, Q., et al. (1996). Cell. 87(5); 905-916).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-LINE1 ORF2, clone 1G4E11, Cat. No. MABE1987, is a mouse monoclonal antibody that detects LINE-1 retrotransposable element ORF2 protein and is tested for use in ELISA, Inhibition Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • ELISA
      • Inhibition
      Application NotesTested applications

      Western Blotting Analysis: A representative lot detected LINE1 ORF2 in Western Blotting applications (Sokolowski, M. et al. (2014). Mob DNA. 5(1):29.).

      Inhibition Analysis: A representative lot partially inhibited L1 endonuclease activity in an in vitro endonuclease cleavage assay. (Sokolowski, M. et al. (2014). Mob DNA. 5(1):29.).

      ELISA Analysis: A representative lot detected LINE1 ORF2 in ELISA applications (Sokolowski, M. et al. (2014). Mob DNA. 5(1):29.).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenA His-tagged recombinant human protein containing an ORF2p endonuclease.
      EpitopeN-terminal
      Clone1G4E11
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1G4E11 is a mouse monoclonal antibody that detects LINE-1 retrotransposable element ORF2 protein (LINE1 ORF2).
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Orf2p
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~32 kDa for truncated recombinant endonuclease domain; 149.01 kDa calculated for full-length protein. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells overexpressing human ORF2p EN.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected LINE1 ORF2 in lysate from HEK293 cells overexpressing human ORF2p EN.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1987-25UL 04065268978962