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MABN2463-25UL Anti-KCNT1 Antibody, clone N3/26

Anti-KCNT1, clone N3/26, Cat. No. MABN2463, is a mouse monoclonal antibody that detects Potassium channel subfamily T member 1 (KCNT1) and is tested for use in Immunohistochemistry (Paraffin) and West

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MABN2463-25UL
25 µL  
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      Overview

      Description
      Catalogue NumberMABN2463-25UL
      DescriptionAnti-KCNT1 Antibody, clone N3/26
      Alternate Names
      • Potassium channel subfamily T member 1
      • Sequence like a calcium-activated potassium channel subunit
      • Slack
      Background InformationPotassium channel subfamily T member 1 (UniProt: Q9Z258; also known as Sequence like a calcium-activated potassium channel subunit, Slack, KCNT1) is encoded by the Kcnt1 (also known as Slack) gene (Gene ID: 60444) in rat. KCNT1 is a multi-pass membrane protein that serves as an outwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. It represents the largest known potassium channel subunit. It is activated by high intracellular sodium or chloride levels and is activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. KCNT1 has four cytoplasmic domains, six transmembrane domains, and four extracellular domains. It is widely expressed in the nervous system and its activity contributes to the slow hyperpolarization that follows repetitive firing. KCNT1 is phosphorylated by protein kinase C and phosphorylation of the C-terminal domain is shown to increase its channel activity by 2 to 3-folds. Mutations in Kcnt1 gene have been linked to malignant migrating partial seizures of infancy (MMPSI), a rare epileptic encephalopathy of infancy. Two isoforms of KCNT1 have been described that are produced by alternative splicing. (Ref.: Barcia, G., et al. (2012). Nat. Gen. 44(11); 1255-1259).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-KCNT1, clone N3/26, Cat. No. MABN2463, is a mouse monoclonal antibody that detects Potassium channel subfamily T member 1 (KCNT1) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected KCNT1 in rat brain tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFusion protein corresponding to 70 amino acids from the C-terminal, cytoplasmic domain of rat Potassium channel subfamily member 1 (KCNT1).
      EpitopeCytoplasmic domain
      CloneN3/26
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone N3/26 is a mouse monoclonal antibody that detects Potassium channel subfamily member 1 (KCNT1). It targets an epitope within 70 amino acids from the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Human, Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Kcnt1
      • Slack
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~140 kDa observed; 139.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected KCNT1 in rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2463-25UL 04065266298499