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ABF998-25UL Anti-IspH

Anti-IspH, Cat. No. ABF998, is a rabbit polyclonal antibody that detects 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (IspH) and is tested for use in ELISA and Western Blotting.

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ABF998-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABF998-25UL
      DescriptionAnti-IspH
      Alternate Names
      • 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
      • EC:1.17.7.4
      • HMBPP reductase
      • 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase
      Background Information4-hydroxy-3-methylbut-2-enyl diphosphate reductase (UniProt: P62623; also known as EC:1.17.7.4, HMBPP reductase, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase) is encoded by the ispH (also known as lytB, yaaE, b0029, JW0027) gene (Gene ID: 944777) in E. coli. IspH is an enzyme that catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). It contains a central iron-sulfur cluster that serves as an electron transfer cofactor in the active site. IspH acts in the terminal step of the methyl erythritol phosphate (MEP) pathway for isoprenoid precursor biosynthesis that is essential for survival of Gram-negative bacteria, mycobacteria, and apicomplexans. It operates at an optimum pH of 7.0. Its substrate binding site is localized in amino acids 225-227. Since MEP pathway is absent in human, inhibitors of IspH are attractive targets for the development of antibiotics. More recently, ester-based prodrug of C23 analog have been described that inhibit IspH and are highly potent against Gram-negative bacteria. (Ref.: Singh, KS., et al. (2021). Nature 589(7843); 597-602; Grawert, T., et al. (2004). J. Am. Chem. Soc. 126(40); 12847-12855).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.02% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IspH, Cat. No. ABF998, is a rabbit polyclonal antibody that detects 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (IspH) and is tested for use in ELISA and Western Blotting.
      Key Applications
      • Western Blotting
      • Enzyme Immunoassay (ELISA)
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected IspH in Western Blotting applications (Singh, K., et al. (2021). Nature. 589(7843):597-602).

      Enzyme Immunoassay Analysis (ELISA): A representative lot detected IspH in ELISA applications (Singh, K., et al. (2021). Nature. 589(7843):597-602).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified E. coli IspH protein was mixed with the KLH conjugate and Freud s complete adjuvant.
      EpitopeUnknown
      Concentration0.30 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (IspH) in E.coli.
      IsotypeIgG
      Species Reactivity
      • E. coli
      Species Reactivity NoteE. Coli.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ispH
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~35 kDa observed; 34.78 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in E. coli lysates of strain CGSC8074 (positive) with conditional knockdown of IspH.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected IspH in lysate from E. coli, strain CGSC8074, but not in lysate from E.coli with conditional knockdown of IspH.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABF998-25UL 04065267216690