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ST1120 Anti-IDE/Insulysin Rabbit pAb

Anti-IDE/Insulysin Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Insulin Degrading Enzyme, Anti-Insulysin

ST1120
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~110 kDa IDE protein in HT1080 cells.
      Catalogue NumberST1120
      Brand Family Calbiochem®
      SynonymsAnti-Insulin Degrading Enzyme, Anti-Insulysin
      Application Data
      Lane 1: Detection of human IDE/insulysin by immunoblotting. Sample: Whole cell lysate (20 µg) from HT1080 cells. Primay antibody: Anti-IDE/Insulysin Rabbit pAb (Cat. No. ST1120) (0.5 µg/ml). Detection: chemiluminescence. Lane 2. Detection of human IDE/insulysin by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HT1080 cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-IDE/Insulysin Rabbit pAb (Cat. No. ST1120) (5 µg). Immunoblotting conditions: same as lane 1.
      References
      ReferencesQiu, W.Q. and Folstein, M.F. 2006. Neurobiol. Aging 27, 190.
      Song, E.H., et al. 2005. J. Biol. Chem. 280, 17701.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlHT1080 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (0.5 µg/ml)
      Immunoprecipitation (5 µg/500 µg total protein)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids at the N-terminus of human IDE/insulysin
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1120 0

      Documentation

      Anti-IDE/Insulysin Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-IDE/Insulysin Rabbit pAb Certificates of Analysis

      TitleLot Number
      ST1120

      References

      Reference overview
      Qiu, W.Q. and Folstein, M.F. 2006. Neurobiol. Aging 27, 190.
      Song, E.H., et al. 2005. J. Biol. Chem. 280, 17701.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-October-2007 RFH
      SynonymsAnti-Insulin Degrading Enzyme, Anti-Insulysin
      ApplicationImmunoblotting (0.5 µg/ml)
      Immunoprecipitation (5 µg/500 µg total protein)
      Application Data
      Lane 1: Detection of human IDE/insulysin by immunoblotting. Sample: Whole cell lysate (20 µg) from HT1080 cells. Primay antibody: Anti-IDE/Insulysin Rabbit pAb (Cat. No. ST1120) (0.5 µg/ml). Detection: chemiluminescence. Lane 2. Detection of human IDE/insulysin by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HT1080 cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-IDE/Insulysin Rabbit pAb (Cat. No. ST1120) (5 µg). Immunoblotting conditions: same as lane 1.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~110 kDa IDE/insulysin protein.
      BackgroundInsulysin or insulin degrading enzyme (IDE; accession no. P14735), also known as insulinase, is evolutionarily conserved, ubiquitously expressed, ~110 kDa zinc metalloprotease. It is located in the cytosol, on the cell surface, and may also be secreted. IDE is the major enzyme responsible for degrading insulin and is, thus, critical for glucose, lipid, and protein metabolism, as well as for cell growth and differentiation. IDE also degrades cerebral Aβ, a protein whose cleaved peptides contribute to the pathology of Alzheimer's disease. Homozygous deletion of the IDE gene in mice results in an increase in the level of Aβ as high as 65%, as well as an increase of 180% in serum insulin levels accompanied by impaired glucose tolerance, which is a hallmark of type 2 diabetes mellitus (DM2). Although hyperinsulinemia and glucose intolerance are the hallmark of DM2, etiology of the disease and the specific role of IDE in this process is not well understood. In human genetic studies, the IDE region of chromosome 10q has shown linkage to late-onset AD, DM2, and fasting plasma glucose level. In a recent study IDE was also identified as a cellular receptor for chickenpox virus (VZV) glycoprotein E, gE, which is involved in viral entry and cell-to-cell spread.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids at the N-terminus of human IDE/insulysin
      IsotypeIgG
      Specieshuman
      Positive controlHT1080 cells
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesQiu, W.Q. and Folstein, M.F. 2006. Neurobiol. Aging 27, 190.
      Song, E.H., et al. 2005. J. Biol. Chem. 280, 17701.