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382158 Anti-Histone H3, Acetylated (1-20) Rabbit pAb

Anti-Histone H3, Acetylated (1-20), rabbit polyclonal, recognizes the ~17 kDa acetylated histone H3 protein. It is validated for use in Western blotting, immunocytochemistry & immunoprecipitation.

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382158
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, TRbPolyclonal Antibody

Pricing & Availability

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382158-50UG
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      		 Plastic ampoule 50 μg
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      Description
      OverviewRecognizes the ~17 kDa acetylated histone H3 protein.
      Catalogue Number382158
      Brand Family Calbiochem®
      Application Data
      Detection of human acetylated histone H3 by immunoblotting. Samples: Acid-extract cells left untreated (lane 1) and sodium butyrate treated (lane 2). Primary antibody: Anti-Histone H3, Acetylated (1-20) Rabbit pAb (Cat. No. 382158) (0.01 µg/ml). Detection: chemiluminescence.
      References
      ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
      Braunstein, M., et al. 1993. Genes Devel. 7, 592.
      Perry, C.A., et al. 1993. Biochemistry 32, 13605.
      Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
      Positive controlHeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (10-500 ng/ml, chemiluminescence)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (see comments)
      Application CommentsFor immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Tetrahymena
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      382158-50UG 04055977213201

      Documentation

      Anti-Histone H3, Acetylated (1-20) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histone H3, Acetylated (1-20) Rabbit pAb Certificates of Analysis

      TitleLot Number
      382158

      References

      Reference overview
      Braunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
      Braunstein, M., et al. 1993. Genes Devel. 7, 592.
      Perry, C.A., et al. 1993. Biochemistry 32, 13605.
      Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-August-2007 RFH
      ApplicationImmunoblotting (10-500 ng/ml, chemiluminescence)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (see comments)
      Application Data
      Detection of human acetylated histone H3 by immunoblotting. Samples: Acid-extract cells left untreated (lane 1) and sodium butyrate treated (lane 2). Primary antibody: Anti-Histone H3, Acetylated (1-20) Rabbit pAb (Cat. No. 382158) (0.01 µg/ml). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa acetylated histone H3 protein.
      BackgroundThe acetylation of core histones regulates gene expression through alterations in the chromatin structure. Lysine acetylation in the amino-terminal domains of histones H3 and H4 can alter the topology of the DNA in chromatin and probably influence its higher-order folding.
      HostRabbit
      Immunogen speciesHuman
      Immunogena diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH
      IsotypeIgG
      SpeciesTetrahymena, human
      Positive controlHeLa cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
      Braunstein, M., et al. 1993. Genes Devel. 7, 592.
      Perry, C.A., et al. 1993. Biochemistry 32, 13605.
      Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.