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368673 Anti-Grp78 (645-654) Rabbit pAb

Anti-Grp78 (645-654) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-BiP

368673
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~78 kDa Grp78 protein.
      Catalogue Number368673
      Brand Family Calbiochem®
      SynonymsAnti-BiP
      Application Data
      Detection of mouse Grp78 by immunoblotting. Samples: Molecular weight marker (lane 1), Grp78 (BiP) recombinant protein (SPP-765) (lane 2), whole cell lysate from CHO-K1 cells (lane 3), and extract from mouse liver microsome (lane 4). Primary antibody: Anti-Grp78 (645-654) Rabbit pAb (Cat. No. 368673) (1:1000). Detection: chemiluminescence.
      References
      ReferencesTing, J., and Lee, A.S. 1989. DNA 7, 275.
      Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
      Warren, G. 1987. Nature 327, 17.
      Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
      Application CommentsThe immunogen sequence is identical to the corresponding sequence in mouse and hamster Gfp78, but cross-reactivity has not been tested. To reduce background by immunoblotting, we recommend blocking with 10% (w/v) skim milk powder in PBS, 0.05% Tween®-20 detergent. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide (EEDTSEKDEL) corresponding to amino acids 645-654 of rat Grp78, conjugated to KLH
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      368673 0

      Documentation

      Anti-Grp78 (645-654) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Grp78 (645-654) Rabbit pAb Certificates of Analysis

      TitleLot Number
      368673

      References

      Reference overview
      Ting, J., and Lee, A.S. 1989. DNA 7, 275.
      Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
      Warren, G. 1987. Nature 327, 17.
      Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-May-2008 JSW
      SynonymsAnti-BiP
      ApplicationImmunoblotting (1 µg/ml, chemiluminescence)
      Application Data
      Detection of mouse Grp78 by immunoblotting. Samples: Molecular weight marker (lane 1), Grp78 (BiP) recombinant protein (SPP-765) (lane 2), whole cell lysate from CHO-K1 cells (lane 3), and extract from mouse liver microsome (lane 4). Primary antibody: Anti-Grp78 (645-654) Rabbit pAb (Cat. No. 368673) (1:1000). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~78 kDa Grp78 protein.
      BackgroundGrp78 is a resident, soluble protein in the ER that is involved in the proper assembly and folding of newly synthesized proteins. Grp78 contains a C-terminal KDEL sequence for retention in the ER
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide (EEDTSEKDEL) corresponding to amino acids 645-654 of rat Grp78, conjugated to KLH
      IsotypeIgG
      Specieshamster, mouse, rat
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe immunogen sequence is identical to the corresponding sequence in mouse and hamster Gfp78, but cross-reactivity has not been tested. To reduce background by immunoblotting, we recommend blocking with 10% (w/v) skim milk powder in PBS, 0.05% Tween®-20 detergent. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesTing, J., and Lee, A.S. 1989. DNA 7, 275.
      Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
      Warren, G. 1987. Nature 327, 17.
      Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.