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MAB3580 Anti-Green Fluorescent Protein Antibody

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MAB3580
100 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AELISA, ICC, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3580
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Green Fluorescent Protein Antibody
      Alternate Names
      • GFP
      • eGFP
      Background InformationThe Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
      Product Information
      FormatPurified
      Control
      • GFP fusion protein expressed in cells
      PresentationLiquid in PBS containing 1 mg/mL BSA. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationDetect Green Fluorescent Protein using this Anti-Green Fluorescent Protein Antibody validated for use in ELISA, IC, IH & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:2,500-1:5,000. Recommended dilution buffer is PBS containing 1 mg/mL BSA.

      Immunocytochemistry: 1:500-1:1,000

      Immunohistochemistry: 1:500-1:1,000 on paraformaldehyde fixed tissues with an overnight incubation using Nickel enhanced DAB for detection. Suggested permeabilization method is 0.5% Triton X-100. Recommended blocking buffer is PBS containing BSA and serum from the host of the secondary antibody. Recommended dilution buffer is PBS containing 1 mg/mL of BSA.

      ELISA: 1:1,000-1:2,000 in a direct assay.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenGFP-BSA
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with Green Fluorescent Protein (GFP). The antibody reacts with various forms of GFP including eGFP including those found in vectors supplied by Clonetech and Invitrogen. There is not cross-reactivity to RFP. YFP has not been tested. This antibody works well for immunohistochemistry.
      IsotypeIgG1
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular WeightMW for GFP is 27 kDa. Dependent upon the molecular weight of the fusion protein being detected.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3580 04053252579530