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PC408 Anti-Green Fluorescent Protein (26-39) Rabbit pAb

Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-GFP

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PC408
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Overview

Replacement Information

Key Spec Table

HostAntibody Type
RbPolyclonal Antibody

Pricing & Availability

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PC408-100UL
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      Description
      OverviewRecognizes the ~28 kDa GFP and some GFP fusion proteins.
      Catalogue NumberPC408
      Brand Family Calbiochem®
      SynonymsAnti-GFP
      References
      ReferencesCubitt, A.B., et al. 1995. Trends Biochem. Sci. 20, 448.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1:2000)
      Immunocytochemistry (1:200)
      Application CommentsThis sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein
      ImmunogenAequora victoria
      HostRabbit
      IsotypeIgG
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC408-100UL 04055977224573

      Documentation

      Anti-Green Fluorescent Protein (26-39) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Green Fluorescent Protein (26-39) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC408

      References

      Reference overview
      Cubitt, A.B., et al. 1995. Trends Biochem. Sci. 20, 448.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-October-2007 RFH
      SynonymsAnti-GFP
      ApplicationImmunoblotting (1:2000)
      Immunocytochemistry (1:200)
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.
      BackgroundGreen Fluorescent Protein (GFP) is responsible for bioluminescence in the jellyfish Aequorea victoria. It has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
      HostRabbit
      Immunogen speciesAequora victoria
      Immunogena synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein
      IsotypeIgG
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCubitt, A.B., et al. 1995. Trends Biochem. Sci. 20, 448.