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GF05 Anti-Granulocyte Colony Stimulating Factor (Ab-1) Mouse mAb (5.24)

Anti-Granulocyte Colony Stimulating Factor (Ab-1) mouse monoclonal, clone 5.24, recognizes ~19 kDa G-CSF in bone marrow & fetal liver tissue. It is validated for use in WB, IF & IHC (paraffin).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-G-CSF

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GF05
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~19 kDa G-CSF glycoprotein in bone marrow and fetal liver tissue.
      Catalogue NumberGF05
      Brand Family Calbiochem®
      SynonymsAnti-G-CSF
      References
      ReferencesNicola, N.A. 1987. J. Cell Cloning 5, 1.
      Metcalf, D., 1986. Blood 67, 257.
      Nagata, S., et al. 1986. Nature 319, 415.
      Nagata, S., et al. 1986 EMBO J. 5, 575.
      Souza, L.M., et al. 1986 Science 232, 61.
      Metcalf, D. 1984, Hemopoietic colony stimulating factors. Amsterdam: Elsevier.
      Nicola, N.A. Vadas, M.A., 1984. Immunol Today 5, 76.
      Nicola, N.A., et al. 1983 J. Biol. Chem. 258, 9017.
      Burgess, A.W., Metcalf, D. 1980. J. Cancer 26, 647.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlRecombinant G-CSF protein, bone marrow tissue, or fetal liver tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Paraffin Sections
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see comments)
      Application CommentsFor paraffin sections, saponin treatment is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified human G-CSF
      ImmunogenHuman
      Clone5.24
      HostMouse
      IsotypeIgG₂
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GF05 0

      Documentation

      Anti-Granulocyte Colony Stimulating Factor (Ab-1) Mouse mAb (5.24) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Granulocyte Colony Stimulating Factor (Ab-1) Mouse mAb (5.24) Certificates of Analysis

      TitleLot Number
      GF05

      References

      Reference overview
      Nicola, N.A. 1987. J. Cell Cloning 5, 1.
      Metcalf, D., 1986. Blood 67, 257.
      Nagata, S., et al. 1986. Nature 319, 415.
      Nagata, S., et al. 1986 EMBO J. 5, 575.
      Souza, L.M., et al. 1986 Science 232, 61.
      Metcalf, D. 1984, Hemopoietic colony stimulating factors. Amsterdam: Elsevier.
      Nicola, N.A. Vadas, M.A., 1984. Immunol Today 5, 76.
      Nicola, N.A., et al. 1983 J. Biol. Chem. 258, 9017.
      Burgess, A.W., Metcalf, D. 1980. J. Cancer 26, 647.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-December-2008 RFH
      SynonymsAnti-G-CSF
      ApplicationImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see comments)
      DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes granulocyte colony stimulating factor.
      BackgroundGranulocyte colony-stimulating factor (G-CSF) belongs to a family of hemopoietic growth factors controlling the survival, proliferation, differentiation and function of granulocytes, macrophages and their precursors. Other members of the family include interleukin-3 or multi-CSF, granulocyte-macrophage-CSF (GM-CSF) and macrophage-CSF (CSF-1). G-CSF was first described as a factor that induces differentiation in a murine myelomonocytic cell line WEHI-3BD and this observation was later confirmed using purified G-CSF. Murine and human G-CSF bind equally well to the receptors of either species and show complete biological cross-reactivity. Biochemically, human G-CSF is a hydrophobic glycoprotein of 19,000 molecular weight with a disperse range of isoelectric points (pI 5.5-6.1) due to glycosylation. The sequence of human cDNA clones isolated by two independent research groups differ only in the presence or absence of a sequence encoding 3 amino acid residues and shows a high degree of homology with murine G-CSF. The origin of the two mRNA species was latter identified as an alternative splice junction at the end of exon 2.
      HostMouse
      Immunogen speciesHuman
      Immunogenpurified human G-CSF
      Clone5.24
      IsotypeIgG₂
      Specieshuman
      Positive controlRecombinant G-CSF protein, bone marrow tissue, or fetal liver tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor paraffin sections, saponin treatment is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesNicola, N.A. 1987. J. Cell Cloning 5, 1.
      Metcalf, D., 1986. Blood 67, 257.
      Nagata, S., et al. 1986. Nature 319, 415.
      Nagata, S., et al. 1986 EMBO J. 5, 575.
      Souza, L.M., et al. 1986 Science 232, 61.
      Metcalf, D. 1984, Hemopoietic colony stimulating factors. Amsterdam: Elsevier.
      Nicola, N.A. Vadas, M.A., 1984. Immunol Today 5, 76.
      Nicola, N.A., et al. 1983 J. Biol. Chem. 258, 9017.
      Burgess, A.W., Metcalf, D. 1980. J. Cancer 26, 647.