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NB37 Anti-Golgi Apparatus Mouse mAb (AE-6)

Anti-Golgi Apparatus Mouse mAb (AE-6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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NB37
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.



      Recognizes the Golgi apparatus in A431 and HeLa cells.
      Catalogue NumberNB37
      Brand Family Calbiochem®
      References
      ReferencesGomord, V., et al. 1999. Biochimie 81, 607.
      Burkhardt, J.K. 1998. Biochim. Biophys. Acta 1404, 113.
      Cabrera-Poch, N., et al. 1998. Biochim. Biophys. Acta 1404, 139.
      Kaiser, C., and Ferro-Novick, S., 1998. Curr Opin Cell Biol. 10, 477.
      Keenan, T.W., 1998. Histochem. Cell Biol. 109, 505.
      La Borgne, R., and Hoflack, B., 1998. Biochim Biophys Acta 1404, 195.
      Munro, S., 1998. Trends Cell Biol. 8, 11.
      Pelham, H. R., 1998. Trends Cell Biol. 8, 45.
      Warren, G., and Malhotra, V., 1998. Curr. Opin. Cell Biol. 10, 4993.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlA-431 or HeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Not Immunoprecipitation
      Not Paraffin Sections
      Application NotesImmunoblotting (2.5 µg/ml)
      Immunofluorescence (1 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenthe Golgi fraction of human cells
      ImmunogenHuman
      CloneAE-6
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NB37 0

      Documentation

      Anti-Golgi Apparatus Mouse mAb (AE-6) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Golgi Apparatus Mouse mAb (AE-6) Certificates of Analysis

      TitleLot Number
      NB37

      References

      Reference overview
      Gomord, V., et al. 1999. Biochimie 81, 607.
      Burkhardt, J.K. 1998. Biochim. Biophys. Acta 1404, 113.
      Cabrera-Poch, N., et al. 1998. Biochim. Biophys. Acta 1404, 139.
      Kaiser, C., and Ferro-Novick, S., 1998. Curr Opin Cell Biol. 10, 477.
      Keenan, T.W., 1998. Histochem. Cell Biol. 109, 505.
      La Borgne, R., and Hoflack, B., 1998. Biochim Biophys Acta 1404, 195.
      Munro, S., 1998. Trends Cell Biol. 8, 11.
      Pelham, H. R., 1998. Trends Cell Biol. 8, 45.
      Warren, G., and Malhotra, V., 1998. Curr. Opin. Cell Biol. 10, 4993.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      ApplicationImmunoblotting (2.5 µg/ml)
      Immunofluorescence (1 µg/ml)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3-X63-Ag8-653 mouse myeloma cells. Recognizes the golgi apparatus protein.
      BackgroundThe golgi compartment occupies a key juxtanuclear area in the cell and has been well defined biochemically. Within the golgi apparatus there is a population of proteins that are maintained that are crucial for specifying localization with the organelle and for specifying the positioning and composition of the golgi itself. The golgi apparatus is responsible for trafficking of proteins through the cell and also serves as a storage site for both soluble and membrane localized proteins. The golgi apparatus is intimately associated with the ER and the microtuble cytoskeleton and the flow between the various compartments is a dynamic environment.
      HostMouse
      Immunogen speciesHuman
      Immunogenthe Golgi fraction of human cells
      CloneAE-6
      IsotypeIgG₁
      Specieshuman, not mouse, not rat
      Positive controlA-431 or HeLa cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesGomord, V., et al. 1999. Biochimie 81, 607.
      Burkhardt, J.K. 1998. Biochim. Biophys. Acta 1404, 113.
      Cabrera-Poch, N., et al. 1998. Biochim. Biophys. Acta 1404, 139.
      Kaiser, C., and Ferro-Novick, S., 1998. Curr Opin Cell Biol. 10, 477.
      Keenan, T.W., 1998. Histochem. Cell Biol. 109, 505.
      La Borgne, R., and Hoflack, B., 1998. Biochim Biophys Acta 1404, 195.
      Munro, S., 1998. Trends Cell Biol. 8, 11.
      Pelham, H. R., 1998. Trends Cell Biol. 8, 45.
      Warren, G., and Malhotra, V., 1998. Curr. Opin. Cell Biol. 10, 4993.