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MAB5406B Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate

This Anti-GAD Antibody, clone 1G10.2, Biotin Conjugate is validated for use in IHC for the detection of GAD67. See below for more information about this anti GAD antibody.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: GAD1, GAD, GAD67 UniProt Number: Q99259
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MAB5406B
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, IHCMBiotinMonoclonal Antibody
      Description
      Catalogue NumberMAB5406B
      DescriptionAnti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate
      Alternate Names
      • Glutamate decarboxylase 1
      • 67 kDa glutamic acid decarboxylase
      • GAD-67
      • Glutamate decarboxylase 67 kDa isoform
      Background InformationGutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, 1999). GAD65 is an amphiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk, 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.
      Product Information
      FormatBiotin
      Control
      • Rat cortex tissue
      PresentationPurified mouse monoclonal IgG2a conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-GAD Antibody, clone 1G10.2, Biotin Conjugate is validated for use in IHC for the detection of GAD67. See below for more information about this anti GAD antibody.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAD67 in rat E18 primary cortex cells.
      Biological Information
      ImmunogenRecombinant GAD67 protein
      Clone1G10.2
      HostMouse
      SpecificityReacts with GAD67. No detectable cross reactivity with GAD65 by Western blot on rat brain lysate.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Rat. Predicted to react with Human and Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GAD1
      • GAD
      • GAD67
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Catalyzes the production of GABA.

      CATALYTIC ACTIVITY: L-glutamate = 4-aminobutanoate + CO2.

      COFACTOR: Pyridoxal phosphate.

      SUBUNIT STRUCTURE: Homodimer.

      TISSUE SPECIFICITY: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.

      INVOLVEMENT IN DISEASE: Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.

      SEQUENCE SIMILARITIES: Belongs to the group II decarboxylase family.
      Molecular Weight~67 kDa.
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in rat cortex tissue.

      Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected GAD67 in rat cortex tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MAB5406B 04053252647765