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MABC128 Anti-FIP200 Antibody, clone 14E11.2

Anti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC.

Anti-FIP200 Antibody, clone 14E11.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: RB1CC1, KIAA0203, RBICC UniProt Number: Q86YR4
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MABC128
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC128
      DescriptionAnti-FIP200 Antibody, clone 14E11.2
      Alternate Names
      • RB1-inducible coiled-coil protein
      • 1FAK family kinase-interacting protein of 200 kDa
      • FIP200
      Background InformationFocal adhesion kinase family interacting protein of 200 kDa (FIP200) is a novel protein inhibitor for focal adhesion kinase (FAK). FIP200 inhibits kinase activity and associated cellular functions, such as cell adhesion, spreading, and motility in fibroblasts by binding directly to FAK.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected FIP200 in serum starved and non-serum starved HeLa cell lysate.

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FIP200 in NIH/3T3, A431, and HeLa cells.

      Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FIP200 in untreated and chloroquinone-treated HUVEC cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the interaction domain of human FIP200.
      EpitopeInteraction domain
      Clone14E11.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody binds to the interaction domain of FIP200, which is needed for the formation of the ULK-FIP200 complex under starved, autophagy conditions.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RB1CC1
      • KIAA0203
      • RBICC
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~200 kDa observed. An uncharacterized band may be observed at ~50 kDa in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected FIP200 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC128 04053252698811

      Documentation

      Anti-FIP200 Antibody, clone 14E11.2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-FIP200 Antibody, clone 14E11.2 Certificates of Analysis

      TitleLot Number
      Anti-FIP200, clone 14E11.2 - 3507302 3507302
      Anti-FIP200, clone 14E11.2 - 3739722 3739722
      Anti-FIP200, clone 14E11.2 - 4010086 4010086
      Anti-FIP200, clone 14E11.2 - 4208491 4208491
      Anti-FIP200, clone 14E11.2 - Q2058408 Q2058408
      Anti-FIP200, clone 14E11.2 -2490007 2490007
      Anti-FIP200, clone 14E11.2 -2621116 2621116
      Anti-FIP200, clone 14E11.2 -2734723 2734723
      Anti-FIP200, clone 14E11.2 -2851237 2851237
      Anti-FIP200, clone 14E11.2 Monoclonal Antibody 2896085
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies