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ST1511 Anti-FGF1 Mouse mAb (3F5)

Anti-FGF1 Mouse mAb (3F5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Fibroblast Growth Factor 1, Acidic

ST1511
  
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      Overview

      Replacement Information

      Key Spec Table

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      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the FGF1 protein in HeLa cells.
      Catalogue NumberST1511
      Brand Family Calbiochem®
      SynonymsAnti-Fibroblast Growth Factor 1, Acidic
      Application Data
      Detection of human FGF1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-FGF1 Mouse mAb (3F5) (Cat. No. ST1511) (1-5 µg/ml). Detection: chemilimunescence.

      Detection of human FGF1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-FGF1 Mouse mAb (3F5) (Cat. No. ST1511) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Imunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 46-155 of human FGF1, expressed as a GST fusion protein
      Clone3F5
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1511 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Fibroblast Growth Factor 1, Acidic
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Imunocytochemistry (10 µg/ml)
      Application Data
      Detection of human FGF1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-FGF1 Mouse mAb (3F5) (Cat. No. ST1511) (1-5 µg/ml). Detection: chemilimunescence.

      Detection of human FGF1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-FGF1 Mouse mAb (3F5) (Cat. No. ST1511) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the FGF1 protein.
      BackgroundFGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 46-155 of human FGF1, expressed as a GST fusion protein
      Clone3F5
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review