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DR1079 Anti-ERCC1 Mouse mAb (3A7)

Anti-ERCC1 Mouse mAb (3A7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Excision Repair Cross-Complementing 1

DR1079
  
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      Overview

      Replacement Information

      Key Spec Table

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      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ERCC1 protein.
      Catalogue NumberDR1079
      Brand Family Calbiochem®
      SynonymsAnti-Excision Repair Cross-Complementing 1
      Application Data
      Detection of human ERCC1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-ERCC1 Mouse mAb (3A7) (Cat. No. DR1079) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 207-282 human ERCC1, expressed as a GST fusion protein
      Clone3A7
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1079 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-Excision Repair Cross-Complementing 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human ERCC1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-ERCC1 Mouse mAb (3A7) (Cat. No. DR1079) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ERCC1 protein.
      BackgroundERCC1 functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light. This protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 207-282 human ERCC1, expressed as a GST fusion protein
      Clone3A7
      IsotypeIgG2a
      Specieshuman
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review