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MABT1487-25UL Anti-E-Cadherin/CD324 Antibody, clone 19A11

Anti-E-Cadherin/CD324, clone 19A11, Cat. No. MABT1487, is a mouse monoclonal antibody that detects E-Cadherin/CD324 and is tested for use as an Agonist and in ELISA, Immunocytochemistry, and Western B

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MABT1487-25UL
25 µL  
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      Overview

      Description
      Catalogue NumberMABT1487-25UL
      DescriptionAnti-E-Cadherin/CD324 Antibody, clone 19A11
      Alternate Names
      • Cadherin-1
      • CAM 120/80
      • Epithelial cadherin
      • Uvomorulin
      • CD324
      Background InformationCadherin-1 (UniProt: P12830; also known as CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324) is encoded by the CDH1 (also known as CDHE, UVO) gene (Gene ID: 999) in human. Cadherins are calcium-dependent cell adhesion molecules that participate in cell-cell adhesion during embryogenesis, development, organogenesis, and differentiation. E-cadherin is a single-pass type I membrane glycoprotein with tumor suppressive properties. It is synthesized with a signal peptide (aa 1-22) and a propeptide (aa 23-154) that are subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 155-709), a transmembrane domain (aa 710-730), and a cytoplasmic domain (aa 731-882). N-glycosylation at Asn-637 is shown to be essential for its expression, folding, and trafficking. E-cadherin is known to contain five cadherin domains. Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Post-translationally it can be cleaved into three chains: E-Cad/CTF1 (aa 701-882); E-Cad/CTF2 (aa 732-882); and E-Cad/CTF3 (aa 751-882). During apoptosis or with calcium influx, it is cleaved by a membrane-bound metalloproteinase (ADAM10; at residues 700-701), which causes disruption of cell-cell adhesion and the subsequent release of β-catenin into the cytoplasm. The residual membrane-tethered cleavage product is then rapidly degraded via an intracellular proteolytic pathway. It can also be cleaved by PS1/ -secretase (at residues 731-732) and this cleavage promotes disassembly of adherens junctions. Clone 19A11 is an activating monoclonal antibody that stimulates adhesion of cells to E-cadherin substrate. It modifies the adhesive properties of E-cadherin and prevent the metastatic invasion of mouse lung cancer cells expressing human E-cadherin. This clone is shown to bind to the extracellular domain of E-cadherin near its primary adhesive motif: the strand swap dimer interface, which strengthens adhesion by stabilizing strand-swap dimers. (Ref.: Xie, B., et al. (2022). Proc. Natl. Acad. Sci. USA. 119 (32); e2204473119; Petrova, YI., et al. (2012). Mol. Biol. Cell. 23(11); 2092-2108; Marambaud, M., et al. (2002). EMBO J. 21(8); 1948-1956; Steinhausen, U., et al. (2001). J. Biol. Chem. 276(7); 4972-4980).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-E-Cadherin/CD324, clone 19A11, Cat. No. MABT1487, is a mouse monoclonal antibody that detects E-Cadherin/CD324 and is tested for use as an Agonist and in ELISA, Immunocytochemistry, and Western Blotting
      Key Applications
      • Immunocytochemistry
      • Agonist or Inhibitor
      • Western Blotting
      • ELISA
      Application NotesTested Applications

      Agonist Function:: A representative lot stimulated adhesion of cells to pure E-Cadherin substrate (Petrova, Y., et al. (2012). Mol Biol Cell.;23(11):2092-108).

      Western Blotting Analysis: A representative lot detected E-Cadherin/CD324) in Western Blotting applications (Petrova, Y., et al. (2012). Mol Biol Cell. 23(11):2092-108; Xie, B., et al. (2022). Proc Natl Acad Sci USA. 119(32):e2204473119).

      Immunocytochemistry Analysis: A representative lot detected E-Cadherin/CD324 in Immunocytochemistry applications (Petrova, Y., et al. (2012). Mol Biol Cell. 23(11):2092-108).

      ELISA Analysis: A representative lot detected E-Cadherin/CD324 in ELISA applications (Petrova, Y., et al. (2012). Mol Biol Cell. 23(11):2092-108).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenRecombinant protein corresponding to the extracellular domain of human E-Cadherin.
      EpitopeN-terminal extracellular domain
      Clone19A11
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 19A11 is a mouse monoclonal antibody that detects E-Cadherin/CD324. It targets an epitope within the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Canine
      Species Reactivity NoteHuman, Canine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDH1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight97.46 kDa calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in MCF-7 cells.

      Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected E-Cadherin/CD324 in MCF-7 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1487-25UL 04065270321688