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ABT266 Anti-Dynein Antibody, HC, cytosolic

This Anti-Dynein Antibody, HC, cytosolic is validated for use in Western Blotting, Immunocytochemistry for the detection of Dynein.

Anti-Dynein Antibody, HC, cytosolic MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: DYNC1H1, CMT2O, DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325, SMALED1 UniProt Number: Q14204
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ABT266
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT266
      DescriptionAnti-Dynein Antibody, HC, cytosolic
      Alternate Names
      • Cytoplasmic dynein 1 heavy chain 1
      • Cytoplasmic dynein heavy chain 1
      • Dynein heavy chain, cytosolic
      Background InformationCytoplasmic dynein 1 heavy chain 1 (UniProt Q14204; also known as Cytoplasmic dynein heavy chain 1, Dynein heavy chain, cytosolic) is encoded by the DYNC1H1 (also known as CMT2O, DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325, SMALED1) gene (Gene ID 1778) in human. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. Cytoplasmic dynein 1 is a multisubunit complex of ∼1.5 MDa composed of a homodimer of heavy chains (encoded by DYNC1H1), two intermediate chains (encoded by DYNC1I1 and DYNC1I2), light-intermediate chains (encoded by DYNC1LI1, DYNC1LI2) and light chains (encoded by DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. The intermediate chain proteins (IC1 & IC2) are involved in cargo binding and specificity, either alone or via interaction with the dynactin complex. IC1 and IC2 share 69% protein identity. They interact with the dynein light chains and the p150 subunit of dynactin at the N-terminus and with the heavy chains through WD40 repeats at the intermediate chain C-terminus.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Dynein Antibody, HC, cytosolic is validated for use in Western Blotting, Immunocytochemistry for the detection of Dynein.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected cytoplasmic dynein heavy chain in HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence within the second AAA domain of human cytoplasmic dynein heavy chain.
      EpitopeSecond AAA domain.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody targets a sequence within the second AAA domain of cytoplasmic dynein heavy chain encoded by the DYNC1H1 gene.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DYNC1H1
      • CMT2O
      • DHC1
      • DNCH1
      • DNCL
      • DNECL
      • DYHC
      • KIAA0325
      • SMALED1
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~500 kDa observed. 532.4 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected cytoplasmic dynein heavy chain in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT266 04055977355390