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MABE1116 Anti-DNAJB1/Hdj1 Antibody, clone J25

This Anti-DNAJB1/Hdj1 Antibody, clone J25 is validated for use in Western Blotting, Immunocytochemistry and Flow Cytometry for the detection of DNAJB1/Hdj1.

Anti-DNAJB1/Hdj1 Antibody, clone J25 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: DNAJ1, DNAJB1, HDJ1, HSPF1 UniProt Number: Q3MI00
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MABE1116
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICC, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1116
      DescriptionAnti-DNAJB1/Hdj1 Antibody, clone J25
      Alternate Names
      • Hdj1
      • DnaJ homolog subfamily B member 1
      • DNAJB1
      • hDj-1
      • Heat shock 40 kDa protein 1
      • Heat shock protein 40
      • HSP40
      Background InformationDnaJ homolog subfamily B member 1 (UniProt Q3MI00) is encoded by the DNAJB1 gene (Gene ID 538426) in bovine species. Heat shock proteins (Hsps) are classified into six major families based on their relative molecular mass, Hsp110/hspH, Hsp90/HspC, Hsp70/HspA, Hsp60/HspD, Hsp40/DnaJ, and small heat shock proteins sHsp/HspB. Hsp40/DnaJB1 belongs to the Hsp40/DnaJ subfamily of heat shock proteins. Hsp40 acts as a co-chaperone with Hsp70 to facilitate protein folding, transport, and degradation. MK5 is reported to stimulate the ATP hydrolyse activity of Hsp40/Hsp70 complex by phosphorylating Hsp40/DnaJB1 C-terminal serine residues Ser149, Ser151, Ser171, resulting in an enhancement of Hsp40/DnaJB1-dependent suppression of heat shock factor 1-driven transcription. DnaJB1 is also shown to interact with MDM2 and prevent MDM2-mediated ubiquitination and proteasomal degradation of p53. DnaJB1 depletion promotes cancer cell growth as a result of p53 pathway inactivation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-DNAJB1/Hdj1 Antibody, clone J25 is validated for use in Western Blotting, Immunocytochemistry and Flow Cytometry for the detection of DNAJB1/Hdj1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesImmunocytochemistry Analysis: 1-5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
      Flow Cytometry Analysis: 1-5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
      Western Blotting Analysis: 0.05-0.5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
      Biological Information
      ImmunogenPurified bovine DNAJB1.
      CloneJ25
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Bovine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DNAJ1
      • DNAJB1
      • HDJ1
      • HSPF1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~38 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human K562 erythroblastoma cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected heat shock-induced DNAJB1/Hdj-1 upregulation in human K-562 erythroleukemia cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1116 04055977296457