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AP1174 Anti-DLC1 Rabbit pAb

AP1174
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~200-250 kDa DLC1 protein.
      Catalogue NumberAP1174
      Brand Family Calbiochem®
      SynonymsAnti-Deleted in Liver Cancer 1, Anti-STARD 12
      Application Data
      Detection of human DLC1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from cells transfected with DLC1. Antibody for IP: Anti-DLC1 Rabbit pAb (Cat. No. AP1174) (ratio of Ab: protein A beads is 1:50-1:100). Antibody for IB: Anti-DLC1 Mouse pAb. Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      PreservativeNone
      Applications
      Key Applications Immunoprecipitation
      Application NotesImmunoprecipitation (see comments)
      Application CommentsFor immunoprecipitation, the ratio of antibody to protein A beads should be 1:50-1:100, but the dilution of antibody needs to be determined for each sample. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, human DLC1 (aa 1-1528)
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1174 0

      Documentation

      Anti-DLC1 Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2010 RFH
      SynonymsAnti-Deleted in Liver Cancer 1, Anti-STARD 12
      ApplicationImmunoprecipitation (see comments)
      Application Data
      Detection of human DLC1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from cells transfected with DLC1. Antibody for IP: Anti-DLC1 Rabbit pAb (Cat. No. AP1174) (ratio of Ab: protein A beads is 1:50-1:100). Antibody for IB: Anti-DLC1 Mouse pAb. Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~200-250 kDa DLC1 protein.
      BackgroundDLC1 is a protein which in humans is encoded by the DLC1 gene. This gene is deleted in the primary tumor of hepatocellular carcinoma. It is suggested that this gene is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal and breast cancers.
      HostRabbit
      Immunogen speciesHuman
      ImmunogenFull-length, human DLC1 (aa 1-1528)
      IsotypeIgG
      Specieshuman
      FormLiquid
      FormulationUndiluted serum.
      PreservativeNone
      CommentsFor immunoprecipitation, the ratio of antibody to protein A beads should be 1:50-1:100, but the dilution of antibody needs to be determined for each sample. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review