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ST1711 Anti-DFHR Mouse mAb (2B10)

This Anti-DFHR Mouse mAb (2B10) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of DFHR.

Anti-DFHR Mouse mAb (2B10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Dihydrofolate Reductase

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ST1711
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, RMMonoclonal Antibody
      Description
      OverviewRecognizes the ~20-22 kDa DHFR protein in HeLa and PC-12 cells.
      Catalogue NumberST1711
      Brand Family Calbiochem®
      SynonymsAnti-Dihydrofolate Reductase
      Application Data
      Detection of human DHFR by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-DFHR Mouse mAb (2B10) (Cat. No. ST1711) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of rat DHFR by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-DFHR Mouse mAb (2B10) (Cat. No. ST1711) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human DHFR by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-DFHR Mouse mAb (2B10) (Cat. No. ST1711) (10 µg/ml). Detection: fluorescence.
      References
      ReferencesVodenicharov, M.D., et al. 2010. The EMBO Journal 29, 3007.
      Ionova, I.A., et al. 2008. Am. J. Physiol. Heart Circ. Physiol. 295 H2178.
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHeLa cells, PC-12 Cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 88-188 human DHFR, expressed as a GST fusion protein
      Clone2B10
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1711 0

      Documentation

      Anti-DFHR Mouse mAb (2B10) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Vodenicharov, M.D., et al. 2010. The EMBO Journal 29, 3007.
      Ionova, I.A., et al. 2008. Am. J. Physiol. Heart Circ. Physiol. 295 H2178.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-Dihydrofolate Reductase
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human DHFR by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-DFHR Mouse mAb (2B10) (Cat. No. ST1711) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of rat DHFR by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-DFHR Mouse mAb (2B10) (Cat. No. ST1711) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human DHFR by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-DFHR Mouse mAb (2B10) (Cat. No. ST1711) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~20-22 kDa DHFR protein.
      BackgroundDihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 88-188 human DHFR, expressed as a GST fusion protein
      Clone2B10
      IsotypeIgG2a
      Specieshuman, rat
      Positive controlHeLa cells, PC-12 Cells
      Negative control293T cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesVodenicharov, M.D., et al. 2010. The EMBO Journal 29, 3007.
      Ionova, I.A., et al. 2008. Am. J. Physiol. Heart Circ. Physiol. 295 H2178.