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MAB3404-C Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free)

This Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free) is validated for use in IHC, western blotting & immunofluorescence for the detection of Cytokeratin 18.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytokeratins Gene Symbol: KRT18, CYK18, PIG46 UniProt Number: P05783
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MAB3404-C
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHC, WB, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3404-C
      Replaces04-586
      DescriptionAnti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free)
      Alternate Names
      • Keratin, type I cytoskeletal 18
      • Cell proliferation-inducing gene 46 protein
      • Cytokeratin-18
      • CK-18
      • Keratin-18
      • K18
      Background InformationCytokeratin 18, also known as Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, CK-18, Keratin-18, or K18, and encoded by the gene KRT18/CYK18/PIG46, is involved in the uptake of thrombin-anti-thrombin complexes by hepatic cells. When the protein is phosphorylated it plays a role in filament reorganization. Cytokeratin-18 together keratin-8 and IL-6 are involved in skin barrier protection too, and cytokeratin-18 is often complexed with Cytokeratin 8. Cytokeratin-18 is expressed in colon, placenta, liver epithelia and many other tissues. Cytokeratin 18 in epithelial cells is cleaved by caspases during apoptosis to produce apoptotic specific CK-18 fragment that is the basis of many apoptosis detection assays.
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free) is validated for use in IHC, western blotting & immunofluorescence for the detection of Cytokeratin 18.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Cytokeratin 18 in human placenta tissue.

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in bladder tissue (Moll, R., et al. (1988). Am. J. Pathol. 132(1): 123-44.).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in ductal carcinoma of the breast, basal cell epithelioma, squamous cell carcinoma of the tongue, squamous cell carcinoma of epiglottis, and squamous cell carcinoma of the esophagus (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 HeLa cell lysate (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).

      Immunofluorescence Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in human epithelia tissue, uterus (cervic glands), submucosal glands of the oesophagus, and placental tissue (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).
      Biological Information
      ImmunogenCytoskeletal preparation of HeLa cells expressing human Cytokeratin 18.
      CloneCK2
      Concentration1 mg/mL
      HostMouse
      SpecificityThe antibody reacts with human cytokeratin 18 and is used for staining research samples of a wide variety of simple epithelia and simple glandular epithelia (e. g. intestinal, respiratory and urinary epithelia) and transitional epithelia (e. g. bladder), but stratified squamous epithelia are not stained (e. g. esophagus, epidermis).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KRT18
      • CYK18
      • PIG46
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~48 kDa calculated
      Product Usage Statements
      Quality AssuranceImmunohistochemistry Analysis: A 1:50 dilution of this antibody detected Cytokeratin 18 in human colorectal cancer tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3404-C 04053252975561