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CP45 Anti-Clathrin Heavy Chain Mouse mAb (X22)

This Anti-Clathrin Heavy Chain Mouse mAb (X22) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of Clathrin Heavy Chain.

Anti-Clathrin Heavy Chain Mouse mAb (X22) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CP45
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, RMMonoclonal Antibody

Pricing & Availability

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CP45-100UL
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      Description
      OverviewRecognizes the ~180 kDa clathrin heavy chain protein.
      Catalogue NumberCP45
      Brand Family Calbiochem®
      References
      ReferencesBauerfeind, R., et al. 1996. J. Neurocytol. 25, 701.
      Zhang, J.Z., et al. 1994. Cell 78, 751.
      Herskovits, J.S., et al. 1993. J. Cell Biol. 122, 565.
      Van Der Bliek, A.M., et al. 1993. J. Cell Biol. 122, 553.
      Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol. 6, 151.
      Brodsky, F.M. 1985. J. Cell Biol. 101, 2047.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (1:500)
      Immunofluorescence (1:1000, indirect)
      Immunoprecipitation (see comments)
      Application CommentsImmunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenclathrin heavy chain purified from human brain
      ImmunogenHuman
      CloneX22
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP45-100UL 04055977220995

      Documentation

      Anti-Clathrin Heavy Chain Mouse mAb (X22) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Clathrin Heavy Chain Mouse mAb (X22) Certificates of Analysis

      TitleLot Number
      CP45

      References

      Reference overview
      Bauerfeind, R., et al. 1996. J. Neurocytol. 25, 701.
      Zhang, J.Z., et al. 1994. Cell 78, 751.
      Herskovits, J.S., et al. 1993. J. Cell Biol. 122, 565.
      Van Der Bliek, A.M., et al. 1993. J. Cell Biol. 122, 553.
      Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol. 6, 151.
      Brodsky, F.M. 1985. J. Cell Biol. 101, 2047.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      ApplicationImmunoblotting (1:500)
      Immunofluorescence (1:1000, indirect)
      Immunoprecipitation (see comments)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 mouse myeloma cells. Recognizes the ~180 kDa clathrin heavy chain protein.
      BackgroundAt the unstimulated presynaptic nerve terminal, a pool of synaptic vesicles remains docked at active zones in preparation for exocytosis. Nerve terminal depolarization induced through an action potential stimulates the rise in intracellular Ca2+, which ultimately initiates the exocytotic process. Fused synaptic vesicles are rapidly replaced with vesicles from the reserve pool, while membranes of the fused vesicles are recaptured by endocytosis. Clathrin is one component involved in vesicle endocytosis, and is composed of three polypeptides, a ~180 kDa heavy chain and two ~32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology that allows clathrin to form its unique polyhedral network. The clathrin-adaptor AP2 and the AP180/AP3 proteins also play a role in this process by forming a protein layer between the membrane and clathrin lattice. The interaction of the AP2 heterotetramer with synaptotagmin is believed to act as the template for recruitment of the AP3 and clathrin triskelia complex. Progression from clathrin-coated to free vesicles in the nerve terminus also requires dynamin.
      HostMouse
      Immunogen speciesHuman
      Immunogenclathrin heavy chain purified from human brain
      CloneX22
      IsotypeIgG₁
      Speciesbovine, human, mouse, rat
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsImmunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBauerfeind, R., et al. 1996. J. Neurocytol. 25, 701.
      Zhang, J.Z., et al. 1994. Cell 78, 751.
      Herskovits, J.S., et al. 1993. J. Cell Biol. 122, 565.
      Van Der Bliek, A.M., et al. 1993. J. Cell Biol. 122, 553.
      Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol. 6, 151.
      Brodsky, F.M. 1985. J. Cell Biol. 101, 2047.