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ABN66 Anti-Cholesterol acyltransferase 1 Antibody

This Anti-Cholesterol acyltransferase 1 Antibody is validated for use in WB, IH(P) for the detection of Cholesterol acyltransferase 1.

Anti-Cholesterol acyltransferase 1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: SOAT1, ACACT, ACACT1, ACAT, ACAT1, SOAT, STAT UniProt Number: P35610
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ABN66
100 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Rhesus Macaque, Chp, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN66
      DescriptionAnti-Cholesterol acyltransferase 1 Antibody
      Alternate Names
      • Sterol O-acyltransferase 1
      • Acyl-coenzyme A:cholesterol acyltransferase 1
      • ACAT-1
      • Cholesterol acyltransferase 1
      Background InformationCholesterol acyltransferase 1 (ACAT1) is mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA and plays a significant role in ketone body metabolism. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Recent studies suggests that ACAT1 may be a therapeutic target for treating certain forms of Alzeheimer's disease.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Caco-2 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Cholesterol acyltransferase 1 Antibody is validated for use in WB, IH(P) for the detection of Cholesterol acyltransferase 1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cholesterol acyltransferase 1 in human adrenal gland and normal human brain tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Cholesterol acyltransferase 1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Rhesus Macaque
      • Chimpanzee
      • Rat
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Rhesus Macaque, Chimpanzee, and Rat based on 100% sequence homology. Other homologies: Mouse (93% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAcyl-coenzyme A:cholesterol acyltransferase (ACACT; EC 2.3.1.26) is an intracellular protein located in the endoplasmic reticulum that forms cholesterol esters from cholesterol. Accumulation of cholesterol esters as cytoplasmic lipid droplets within macrophages and smooth muscle cells is a characteristic feature of the early stages of atherosclerotic plaques (Cadigan et al., 1988).
      Gene Symbol
      • SOAT1
      • ACACT
      • ACACT1
      • ACAT
      • ACAT1
      • SOAT
      • STAT
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase.

      CATALYTIC ACTIVITY: Acyl-CoA + cholesterol = CoA + cholesterol ester.

      SUBUNIT STRUCTURE: May form homo- or heterodimers.

      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.

      INDUCTION: Highly activated by the presence of cholesterol.

      SEQUENCE SIMILARITIES: Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
      Molecular Weight~58 kDa observed which may be the result of dimerization/aggregation of the native protein during sample preparation for SDS-PAGE. The calculated molecular weight is 65 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Caco-2 cell lysate.

      Western Blot Analysis: 0.05 µg/mL of this antibody detected Cholesterol acyltransferase 1 on 10 µg of Caco-2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN66 04053252592041

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anti-Cholesterol acyltransferase 1 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cholesterol acyltransferase 1 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Cholesterol acyltransferase 1 - 3229433 3229433
      Anti-Cholesterol acyltransferase 1 - 3386432 3386432
      Anti-Cholesterol acyltransferase 1 - 3519822 3519822
      Anti-Cholesterol acyltransferase 1 - 3638907 3638907
      Anti-Cholesterol acyltransferase 1 - 3829458 3829458
      Anti-Cholesterol acyltransferase 1 - NRG1920078 NRG1920078
      Anti-Cholesterol acyltransferase 1 -2567123 2567123
      Anti-Cholesterol acyltransferase 1 Polyclonal Antibody 3776145
      Anti-Cholesterol acyltransferase 1 Polyclonal Antibody 2900036