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219010 Anti-Catalase Rabbit pAb

Anti-Catalase, rabbit polyclonal, recognizes the ~60-65 kDa human and mouse catalase. It is validated for ELISA, Western blotting, and immunoelectrophoresis.

Anti-Catalase Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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219010
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Pricing & Availability

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219010-1ML
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      Description
      OverviewRecognizes the ~60-65 kDa catalase protein.
      Catalogue Number219010
      Brand Family Calbiochem®
      Application Data
      Detection of human catalase by immunoblotting. Samples: Low molecular weight markers (lanes 1, 5a, 5b, 9), catalase protein (0.075 µg) (lanes 2 and 6), catalase protein (0.15 µg) (lanes 3 and 7), and catalase protein (0.3 µg) (lanes 4 and 8), and red blood cell lysate (lane 10). With primary antibody dilution 1:8000 (lanes 1-5a) and 1:6000 (lanes 5b-10). Primary antibody: Anti-Catalase Rabbit pAb (Cat. No. 219010) (1:6000). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlRed blood cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting, Paraffin Sections Meilhac, O., et al. 2001. Arterioscler. Thromb. Vasc. Biol. 21, 1681.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoelectrophoresis
      Application NotesIndirect ELISA (1:128)
      Immunoblotting (1:8000; see application references)
      Immunoelectrophoresis (see comments)
      Paraffin Sections (see application references)
      Application CommentsThe 256 kDa catalase protein is a tetramer of 4 identical ~60-65 kDa protein. Exhibits a single arc by immunoelectrophoresis (IEP) vs. human red cell lysate. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenpurified, human erythrocytes catalase
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      219010-1ML 04055977202045

      Documentation

      Anti-Catalase Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Catalase Rabbit pAb Certificates of Analysis

      TitleLot Number
      219010

      Citations

      Title
    • Trine Nilsen, et al. (2007) A nuclear export sequence located on a β-strand in fibroblast growth factor-1. Journal of Biological Chemistry 282, 26245-26256.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      ApplicationIndirect ELISA (1:128)
      Immunoblotting (1:8000; see application references)
      Immunoelectrophoresis (see comments)
      Paraffin Sections (see application references)
      Application Data
      Detection of human catalase by immunoblotting. Samples: Low molecular weight markers (lanes 1, 5a, 5b, 9), catalase protein (0.075 µg) (lanes 2 and 6), catalase protein (0.15 µg) (lanes 3 and 7), and catalase protein (0.3 µg) (lanes 4 and 8), and red blood cell lysate (lane 10). With primary antibody dilution 1:8000 (lanes 1-5a) and 1:6000 (lanes 5b-10). Primary antibody: Anti-Catalase Rabbit pAb (Cat. No. 219010) (1:6000). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation and DEAE-cellulose ion exchange chromatography. Recognizes the ~60-65 kDa (monomer MW) catalase protein under reducing conditions.
      HostRabbit
      Immunogen speciesHuman
      Immunogenpurified, human erythrocytes catalase
      IsotypeIgG
      Specieshuman, mouse
      Positive controlRed blood cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe 256 kDa catalase protein is a tetramer of 4 identical ~60-65 kDa protein. Exhibits a single arc by immunoelectrophoresis (IEP) vs. human red cell lysate. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      Citation
    • Trine Nilsen, et al. (2007) A nuclear export sequence located on a β-strand in fibroblast growth factor-1. Journal of Biological Chemistry 282, 26245-26256.
      		•
      Application referencesImmunoblotting, Paraffin Sections Meilhac, O., et al. 2001. Arterioscler. Thromb. Vasc. Biol. 21, 1681.