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MAB131872 Anti-Cas9 Antibody, clone 6F7

Anti-Cas9, clone 6F7, Cat. No. MAB131872, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunocytochemistry and Western Blotting.

Anti-Cas9 Antibody, clone 6F7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Cas9 UniProt Number: J7RUA5
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MAB131872
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB131872
      DescriptionAnti-Cas9 Antibody, clone 6F7
      Alternate Names
      • CRISPR-associated endonuclease Cas9
      • SaCas9
      Background InformationCRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9, Cas9) is encoded by the Cas9 gene in Streptococcus aureus. Cas9 belongs to subtype II-A subfamily with one HNH Cas9-type domain. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bilobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. The homologous region of the Streptococcus aureus is not closely related in amino acid sequence in S. aureus, hence this antibody does not recognize that protein.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.03% sodium azide and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cas9, clone 6F7, Cat. No. MAB131872, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells which overexpress fusion protein containing GFP and C-terminus of Cas9 from S. aureus (Courtesy of Dr Gerry Shaw).

      Western Blotting Analysis: A representative lot detected Cas9 in HEK293 overexpressing fusion protein containing GFP and C-terminus of Cas9 from S. aureus (Courtesy of Dr Gerry Shaw).
      Biological Information
      ImmunogenA recombinant fragement corresponding to 251 amino acids from the C-terminal region of Staphylococcus aureus. Expressed in and purified from E. coli.
      EpitopeC-terminus
      Clone6F7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6F7 specifically detects Cas9 in Staphylococcus aureus. It does not recognize Cas9 in Streptococcus pyogenes.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteStaphylococcus aureus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Cas9
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~53 kDa observed (truncated form). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysates overexpressing a fusion protein containing GFP and C-terminus of Cas9 from Staphylococcus aureus.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cas9 in 10 µg of HEK293 cell lysates overexpressing a fusion protein containing GFP and c-terminus of Cas9 from Staphylococcus aureus.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB131872 04054839178931