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MABF917 Anti-CRACC Antibody, clone 4G2

Anti-CRACC Antibody, clone 4G2 is an antibody against CRACC for use in Flow Cytometry, Affects Function, Immunoprecipitation.

Anti-CRACC Antibody, clone 4G2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunological Signaling Gene Symbol: Slamf7, Cs1 UniProt Number: Q8BHK6
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MABF917
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, FUNC, IPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF917
      DescriptionAnti-CRACC Antibody, clone 4G2
      Alternate Names
      • SLAM family member 7
      • CD2-like receptor-activating cytotoxic cells
      • CRACC
      • Leukocyte cell-surface antigen
      • Novel Ly9
      • CD319
      Background InformationSLAM family member 7 (UniProt Q8BHK6; also known as CD2-like receptor-activating cytotoxic cells, CRACC, Leukocyte cell-surface antigen, Novel Ly9, CD319) is encoded by the Slamf7 (also known as Cs1) gene (Gene ID 75345) in murine species. CRACC/CS1 belongs to the 6-member (SLAM/CD150, Ly-9/CD229, 2B4/CD244, CD84, NTB-A/CD352, CRACC) signaling lymphocytic activation molecule family (SLAMF) of receptors broadly expressed in hematopoietic cells. 2B4 and CD48 interact with each other, while rest of the family members act as self-ligands, where two identical molecules on the surface of two different cells can interact as receptor and ligand to each other. CRACC is synthesized with a signal peptide sequence (a.a. 1-22), the removal of which yields the mature CRACC with an extracellular region (a.a. 23-224) that contains a V-type and a C2-type Ig-like domain (a.a. 27-112 and 128-203), followed by a single transmembrane domain (a.a. 225-245) and a cytoplasmic tail (a.a. 246-333). CRACC cytoplasmic domain contains one immunoreceptor tyrosine-based switch motif (ITSM; 278-TFYSTV-283, numbering based on mature murine sequence), which upon phosphorylation (pY300) recruits SAP family adaptor EWS-Fli1-activated transcript-2 (EAT-2) for signaling transduction. CRACC can also be phosphorylated on Tyr280. Unlike pY300, pY280 mediates the inhibitory function of CRACC in NK cells. In addition to NK cells, evidence indicates that CRACC is also involved in regulating B- and T-cell functions.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG1κ antibody in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CRACC Antibody, clone 4G2 is an antibody against CRACC for use in Flow Cytometry, Affects Function, Immunoprecipitation.
      Key Applications
      • Flow Cytometry
      • Affects Function
      • Immunoprecipitation
      Application NotesFlow Cytometry Analysis: A representative lot immunostained murine T lymphoma BI-141 transfectants expressing mouse CRACC but not those transfected with other members of the SLAM family (CD84, 2B4, Ly9, Ly108 or SLAM) (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
      Flow Cytometry Analysis: A representative lot detected CRACC expression on the surface of many types of mouse immune system cells, including NK cells, NKT cells, B cells, DCs, macrophages and activated T cells (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
      Affects Function: A representative lot, in combination with a rabbit anti-rat IgG, cross-linked cell surface CRACC and induced similar CRACC phosphorylation in IL-2-activated NK cells from wild-type, SAP-deficient, or EAT-2–ERT–double knock (DKO) mice (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
      Immunoprecipitation Analysis: A representative lot, in combination with a rabbit anti-rat IgG and protein A, immunoprecipitated CRACC from lysates of IL-2-activated mouse NK cells (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
      Biological Information
      ImmunogenHuman IgG1 Fc -tagged recombinant mouse CRACC extracellular domain.
      EpitopeExtracellular domain.
      Clone4G2
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 4G2 reacted with murine T lymphoma BI-141 transfectants expressing mouse CRACC but not with those transfected with other members of the SLAM family (CD84, 2B4, Ly9, Ly108 or SLAM) (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305). Expected to react with all four spliced isoforms of murine CRACC reported by UniProt (Q8BHK6).
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Slamf7
      • Cs1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight34.77 kDa (isoform 1), 34.87 kDa (isoform 2), 30.71 kDa (isoform 3), and 30.61 kDa (isoform 4) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in mouse splenocytes.

      Flow Cytometry Analysis: 0.1 µg of this antibody detected CRACC-positive mouse splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF917 04055977349627