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ABS1668 Anti-CHMP3

Anti-CHMP3, Cat. No. ABS1668, is a rabbit polyclonal antibody that detects Charged multivesicular body protein 3 and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-CHMP3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: CHMP3, CGI149, NEDF, VPS24, CGI-149 UniProt Number: Q9Y3E7
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ABS1668
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1668
      DescriptionAnti-CHMP3
      Alternate Names
      • Charged multivesicular body protein 3
      • Chromatin-modifying protein 3
      • Neuroendocrine differentiation factor
      • Vacuolar protein sorting-associated protein 24
      • hVps24
      Background InformationCharged multivesicular body protein 3 (UniProt: Q9Y3E7; also known as Chromatin-modifying protein 3, CHMP3, Neuroendocrine differentiation factor, Vacuolar protein sorting-associated protein 24, hVps24) is encoded by the CHMP3 (also known as CGI149, NEDF, VPS24, CGI-149) gene (Gene ID: 51652) in human. CHMP3 is a component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multi-vesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. It is widely expressed and its expression is reported in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. The acidic C-terminus and the basic N-terminus of CHMP3 are thought to render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. CHMP3 is shown to heteromerize with CHMP2A to form helical tubular structures that expose membrane-interacting sites on the outside. It can also interact with CHMP4A, however, the interaction requires the release of CHMP4A auto-inhibition.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CHMP3, Cat. No. ABS1668, is a rabbit polyclonal antibody that detects Charged multivesicular body protein 3 and has been tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected human recombinant charged multivesicular body protein 3 (CHMP3).

      Western Blotting Analysis: A representative lot detected CHMP3 in Western Blotting applications (Morita, E., et. al. (2010). Proc Natl Acad Sci USA. 107(29):12889-94).
      Biological Information
      ImmunogenA full-length human recombinant charged multivesicular body protein 3 (CHMP3).
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Charged multivesicular body protein 3 in human cells.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CHMP3
      • CGI149
      • NEDF
      • VPS24
      • CGI-149
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~32 kDa observed; 25.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CHMP3 in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1668 04054839323355