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MABN1524 Anti-CHCHD10 Antibody, clone 11F11.2

Anti-CHCHD10 antibody, clone 11F11.2, Cat. No. MABN1524, is a highly specific mouse monoclonal antibody, that targets CHCHD10 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-CHCHD10 Antibody, clone 11F11.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: CHCHD10, C22orf16 UniProt Number: Q8WYQ3
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MABN1524
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1524
      DescriptionAnti-CHCHD10 Antibody, clone 11F11.2
      Alternate Names
      • Coiled-coil-helix-coiled-coil-helix domain-containing protein 10
      • mitochondrial
      • Protein N27C7-4
      Background InformationCoiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial (UniProt: Q8WYQ3; also known as Protein N27C7-4) is encoded by the CHCHD10 (also known as C22orf16) gene (Gene ID: 400916) in human. CHCHD10 belongs to a family of mitochondrial proteins located in the intermembrane space that is enriched at cristae junctions. CHCHD10 displays a ubiquitous distribution pattern, but its expression is highest in heart and liver and lowest in spleen. It is involved in the maintenance of mitochondrial organization, mitochondrial cristae structure, and mitochondrial DNA stability. CHCD10 has a non-structured N-terminal region, followed by a highly hydrophobic helix and a C-terminal CHCH domain characterized by a Cx(9)C motif and 2 additional cysteines. The four cysteine residues (102, 112, 122 and 132) of the CHCH domain are involved in two disulphide bonds. Overexpression of a CHCHD10 mutant allele in HeLa cells is reported to lead to fragmentation of the mitochondrial network and ultrastructural major abnormalities including loss, disorganization and dilatation of cristae. Mutations in CHCHD10 gene are reported to cause frontotemporal dementia and/or amyotrophic lateral sclerosis 2 (FTDALS2). (Ref.: Bannwarth, S., et al. (2014). Brain. 2014 137(Pt 8):2329-2345).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CHCHD10 antibody, clone 11F11.2, Cat. No. MABN1524, is a highly specific mouse monoclonal antibody, that targets CHCHD10 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected CHCHD10 in 10 µg of human liver tissue lysate.

      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHCHD10 in human small intestine, human tonsil, human liver, and human prostate cancer tissue.
      Biological Information
      ImmunogenGST-tagged recombinat fragment corresponding to 79 amino acids from human CHCHD10.
      Clone11F11.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 11F11.2 detects CHCHD10 in human brain and liver.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CHCHD10
      • C22orf16
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~15 kDa observed; 14.15 kDa calulated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CHCHD10 in 10 µg of human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1524 04054839153167