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MABS2157-25UG Anti-CH25H Antibody, clone 18G9

Anti-CH25H, clone 18G9, Cat. No. MABS2157, is a mouse monoclonal antibody that detects Cholesterol 25-hydroxylase (CH25H) and is tested for use in ELISA and Western Blotting.

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MABS2157-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2157-25UG
      DescriptionAnti-CH25H Antibody, clone 18G9
      Alternate Names
      • EC:1.14.99.38
      • Cholesterol 25-monooxygenase
      • h25OH
      Background InformationCholesterol 25-hydroxylase (UniProt: O95992; also known as EC:1.14.99.38, Cholesterol 25-monooxygenase, h25OH) is encoded by the CH25H gene (Gene ID: 9023) in human. CH25H is a multi-pass membrane glycoprotein with three transmembrane domains (aa 38-58; 84-104; and 121-141) that catalyzes the formation of 25-hydroxycholesterol from cholesterol, which in turn leads to repression of cholesterol biosynthesis. It is also reported to play a role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H is an interferon inducible enzyme that has broad-spectrum antiviral functions. Stimulation of macrophage Toll-like receptors (TLR) is also reported to increase its expression and synthesis of 25-hydroxycholesterol. Treatment of naïve B cells with nanomolar concentrations of 25-hydroxycholesterol is shown to suppress IL-2-mediated stimulation of B cell proliferation, repress activation induced cytidine deaminase expression, and reduce IgA production. Deletion of CH25H gene in mouse is shown to increase in serum IgA. In testis tissue, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. (Ref.: Bauman, DR., et al. (2009). Proc. Natl. Acad. Sci. USA. 106(39); 16764-16769).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ200
      Applications
      ApplicationAnti-CH25H, clone 18G9, Cat. No. MABS2157, is a mouse monoclonal antibody that detects Cholesterol 25-hydroxylase (CH25H) and is tested for use in ELISA and Western Blotting.
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesTested Applicaitons

      Western Blotting Analysis: A representative lot detected CH25H in Western Blotting applications (Bauman, D.R., et al. (2009). Proc Natl Acad Sci USA. 106(39):16764-9; Tuong, Z.K., et. al. (2016). PLoS One. 11(1):e0147179).

      ELISA Analysis: A representative lot detected CH25H in ELISA applications (Bauman, D.R., et al. (2009). Proc Natl Acad Sci USA. 106(39):16764-9).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged full length recombinant human Cholesterol 25-hydroxylase.
      EpitopeUnknown
      Clone18G9
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 18G9 is a mouse monoclonal antibody that detects Cholesterol 25-hydroxylae (CH25H).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CH25H
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~34 kDa observed; 31.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293T cells overexpressing cholesterol 25-hydroxylase (CH25H).

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected cholesterol 25-hydroxylase (CH25H) in lysate from HEK293T cells overexpressing cholesterol 25-hydroxylase (CH25H).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2157-25UG 04065267780665