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MABE1114 Anti-CENP-C Antibody, clone AL61A

Anti-CENP-C Antibody, clone AL61A is an antibody against CENP-C for use in Immunocytochemistry, Immunoprecipitation.

Anti-CENP-C Antibody, clone AL61A MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: CENPC, CENPC1, ICEN7 UniProt Number: Q03188
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MABE1114
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1114
      DescriptionAnti-CENP-C Antibody, clone AL61A
      Alternate Names
      • Centromere protein C
      • CENP-C
      • CENP-C 1
      • Centromere autoantigen C
      • Centromere protein C 1
      • Interphase centromere complex protein 7
      Background InformationCentromere protein C (UniProt Q03188; also known as CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7) is encoded by the CENPC (also known as CENPC1, ICEN7) gene (Gene ID 1060) in human. Kinetochores assemble on centromeres to bind spindle microtubules and mediate chromosome segregation during cell division. CENP-C is a subunit of constitutive centromere-associated network (CCAN) that acts as the centromere-kinetochore interface. CCAN is a large complex composed of at least 16 different centromeric proteins (CENPs). CENP-C plays an important role in kinetochore assembly downstream of CENP-A. The PEST domain in the N-terminal half of CENP-C interacts directly with the CCAN subcomplex CENP-HIKM (CENP-H, CENP-K, CENP-I, and CENP-M) and CENP-C depletion is shown to casue mislocalization of CENP-T and the CENP-HIKM complex subunits in HeLa cells.
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CENP-C Antibody, clone AL61A is an antibody against CENP-C for use in Immunocytochemistry, Immunoprecipitation.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant immunoprecipitated CENP-C from HeLa nuclear extract (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
      Immunocytochemistry Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant detected nuclear CENP-C puncta by fluorescent immunocytochemistry staining of permeabilized HeLa and Jurkat cells (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
      Immunocytochemistry Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant immunostained CENP-C-transfected, but not ZBTB32-transfected, HEK293T cells (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
      Biological Information
      ImmunogenGST-tagged recombinant human CENP-C N-terminal fragment.
      EpitopeN-terminal.
      CloneAL61A
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with both spliced isoforms of human CENP-C reported by UniProt (Q03188).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CENPC
      • CENPC1
      • ICEN7
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight106.8 kDa (isoform 1) and 61.54 kDa (isoform 2) calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in Jurkat cells.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected CENP-C in Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1114 04055977333299